LOCUS       Exported                7804 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pTRK1-PRslpA, complete sequence.
ACCESSION   KC875822
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Fujita A, Misumi Y, Honda S, Sato T, Koyama Y.
  TITLE     Construction of new cloning vectors that employ the phytoene 
            synthase encoding gene for color screening of cloned DNA inserts in 
            Thermus thermophilus
  JOURNAL   Gene (2013) In press
  PUBMED    23845779
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Fujita A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2013) Health Research Institute, National 
            Institute of Advanced Science and Technology, 1-8-31 Midorigaoka, 
            Ikeda, Osaka 563-8577, Japan
REFERENCE   3  (bases 1 to 7804)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Shuttle vector pTRK1-PRslpA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1385595"
     regulatory      168..667
                     /regulatory_class="promoter"
                     /note="slpA promoter"
     misc_feature    673..677
                     /label=cloning site EcoRI
                     /note="cloning site EcoRI"
     misc_feature    684..689
                     /label=cloning site HindIII
                     /note="cloning site HindIII"
     CDS             complement(1519..2682)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="putative RepA protein"
                     /protein_id="AGT36878.1"
                     /translation="MVLRAYAALRGLSPEALRAHLLAPPLRPERAREAFQRPYLAHFAQ
                     TLPRYPYATDDPKEGVRIYKRENALKRVHVQVGHYPHAVLRLVVDVDLPWPQVEERIHA
                     LPPSLVLVNPRSGHFHAWYELDPIPLTPPPGREGSLKGALALLAEVEALLEAYYGADPG
                     YNGLLSRNPFLHPPEWTWGGGKRWSLRDLHRELRGLLPSGTRRRVDPGLASYGRNNALF
                     DRLRAEAYAHVALFRGVPGGEEAFRAWVEQRAHALNQSLFRDHPKGPLDPREVHHTAKS
                     VAKWTYRNYRGARVYPVSSTGRPDRSRLSPQARALIPPLQGQELQEAVREGGRRRGSRR
                     RQEAEEKLTEALKRLQARGERVTARALAREAGVKPHTASKWLKRMRE"
     gene            complement(1519..2682)
                     /gene="repA"
                     /label=repA
     CDS             5174..5944
                     /codon_start=1
                     /gene="KNT"
                     /product="kanamycin nucleotidyltransferase"
                     /label=KNT
                     /protein_id="AGT36879.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHG
                     RFFSILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSD
                     PEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            5174..5944
                     /gene="KNT"
                     /label=KNT
     CDS             6003..6863
                     /codon_start=1
                     /gene="Amp-R"
                     /product="beta-lactamase"
                     /label=Amp-R
                     /protein_id="AGT36880.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            6003..6863
                     /gene="Amp-R"
                     /label=Amp-R
     rep_origin      7034..7622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"