LOCUS       Exported                8796 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRK1-PRslpA-crtBcas, complete sequence.
ACCESSION   KC875823
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Fujita A, Misumi Y, Honda S, Sato T, Koyama Y.
  TITLE     Construction of new cloning vectors that employ the phytoene 
            synthase encoding gene for color screening of cloned DNA inserts in 
            Thermus thermophilus
  JOURNAL   Gene (2013) In press
  PUBMED    23845779
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Fujita A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2013) Health Research Institute, National 
            Institute of Advanced Science and Technology, 1-8-31 Midorigaoka, 
            Ikeda, Osaka 563-8577, Japan
REFERENCE   3  (bases 1 to 8796)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Cloning vector pTRK1-PRslpA-crtBcas"
                     /mol_type="other DNA"
                     /label=T. thermophilus-E. coli shuttle vector
                     /note="T. thermophilus-E. coli shuttle vector"
                     /db_xref="taxon:1385596"
     regulatory      145..644
                     /regulatory_class="promoter"
                     /note="slpA promoter"
     misc_feature    650..655
                     /label=cloning site EcoRI
                     /note="cloning site EcoRI"
     misc_feature    654..659
                     /label=cloning site XbaI
                     /note="cloning site XbaI"
     CDS             660..1529
                     /codon_start=1
                     /gene="crtB"
                     /product="phytoene synthase"
                     /label=crtB
                     /protein_id="AGT36881.1"
                     /translation="MKMPASMEPDWKALLRVLRAHSATFYLGSLLFPKEARKGAWAVYA
                     ACRLGDEAVDGEGGGPEALEAWWAGVERAYRGRPLAEWEKGLAWALERWDIPFEAFLHM
                     REGFLTDLGPVRLGTEAELLRYCYQVAGTVGRMMAPIAGGGKEAEARAVKLGQAMQLTN
                     ILRDVGEDLERDRVYLPLDLLRAHGVEVEDLRAGRVTPGYRALMAHLEGKARALYREGL
                     AGLGHLKVGRAAIALAALQYRGILDKLRLSGYDNLGRRAHLKAWERALLLPKAFLAARF
                     PPRPEGSP"
     gene            660..1529
                     /gene="crtB"
                     /label=crtB
     misc_feature    1265..1270
                     /label=cloning site Acc65I
                     /note="cloning site Acc65I"
     misc_feature    1331..1336
                     /label=cloning site AvrII
                     /note="cloning site AvrII"
     CDS             complement(2511..3674)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /protein_id="AGT36882.1"
                     /translation="MVLRAYAALRGLSPEALRAHLLAPPLRPERAREAFQRPYLAHFAQ
                     TLPRYPYATDDPKEGVRIYKRENALKRVHVQVGHYPHAVLRLVVDVDLPWPQVEERIHA
                     LPPSLVLVNPRSGHFHAWYELDPIPLTPPPGREGSLKGALALLAEVEALLEAYYGADPG
                     YNGLLSRNPFLHPPEWTWGGGKRWSLRDLHRELRGLLPSGTRRRVDPGLASYGRNNALF
                     DRLRAEAYAHVALFRGVPGGEEAFRAWVEQRAHALNQSLFRDHPKGPLDPREVHHTAKS
                     VAKWTYRNYRGARVYPVSSTGRPDRSRLSPQARALIPPLQGQELQEAVREGGRRRGSRR
                     RQEAEEKLTEALKRLQARGERVTARALAREAGVKPHTASKWLKRMRE"
     gene            complement(2511..3674)
                     /gene="repA"
                     /label=repA
     CDS             6166..6936
                     /codon_start=1
                     /gene="KNT"
                     /product="kanamycin nucleotidyltransferase"
                     /label=KNT
                     /protein_id="AGT36883.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
                     QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHG
                     RFFSILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTT
                     FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSD
                     PEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            6166..6936
                     /gene="KNT"
                     /label=KNT
     CDS             6995..7855
                     /codon_start=1
                     /gene="Amp-R"
                     /product="beta-lactamase"
                     /label=Amp-R
                     /protein_id="AGT36884.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            6995..7855
                     /gene="Amp-R"
                     /label=Amp-R
     rep_origin      8026..8614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"