LOCUS       Exported                3589 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTriplEx2, complete sequence.
ACCESSION   EU126551
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Kalnine N.
  TITLE     pTriplEx2 - cDNA library construction vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Kalnine N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2007) Informatics, Clontech, 1290 Terra Bella 
            Ave., Mountain View, CA 94043, USA
REFERENCE   3  (bases 1 to 3589)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Cloning vector pTriplEx2"
                     /mol_type="other DNA"
                     /db_xref="taxon:469761"
     regulatory      94..177
                     /regulatory_class="promoter"
                     /note="lac promoter"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      238..257
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase promoter"
     promoter        238..256
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5UTR           276..413
                     /label=derived from Escherichia coli ompA
                     /note="derived from Escherichia coli ompA"
     primer_bind     457..473
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             469..852
                     /codon_start=1
                     /product="beta-galactosidase a-peptide"
                     /label=beta-galactosidase a-peptide
                     /note="derived from Escherichia coli beta-galactosidase 
                     a-peptide"
                     /protein_id="ABV25015.1"
                     /translation="MTMITPSSEIWTSFFFFSREARHCVGTREFGHYGLQDPAASAQST
                     LDSSKLMHAAAIRAHLANSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWR
                     NSEEARTDRPSQQLRSLNGEWKL"
     misc_feature    529..665
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      661..680
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(687..703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(844..1299)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_recomb     1359..1392
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    1359..1392
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        1683..1787
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1788..2648
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="ABV25014.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2819..3407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"