LOCUS       Exported                5398 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTransgene, complete sequence.
ACCESSION   AF515846
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Yang X, Pachikara B, Murray TJ, Liu D, Sonenshein GE, Sherr DH.
  TITLE     pTransgene: A cloning vector with insulators for optimal cDNA 
            transgene expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Yang X, Sonenshein GE, Sherr DH.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2002) Environmental Health, Boston University 
            School of Public Health and Medicine, 715 Albany Street, R-405, 
            Boston, MA 02118, USA
REFERENCE   3  (bases 1 to 5398)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Cloning vector pTransgene"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:198113"
     misc_feature    1..56
                     /note="BglII, NheI, XhoI and EcoRI restriction sites to 
                     isolate transgene construct"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    62..635
                     /note="chicken beta-globin core insulator; shields 
                     transgene from chromosomal positional effects"
     misc_feature    647..707
                     /note="PacI and AvrII restriction sites for changing 
                     promoter"
     regulatory      668..1948
                     /regulatory_class="promoter"
                     /note="mouse mammary tumor virus 5 LTR"
     misc_feature    1949..1960
                     /note="SacII and SpeI restriction sites for changing 
                     promoter"
     intron          1961..2145
                     /note="chimeric intron:5-donor site is from the 1st intron
                     of the human beta-globin gene, the branch and 3-acceptor 
                     site is from the intron that is between the leader and the 
                     body of an immunoglobulin gene heavy chain variable region;
                     increases the level of transgene expression"
     intron          1986..2118
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    2146..2181
                     /note="multiple cloning site; contains MluI, XbaI, SalI, 
                     AccI, EagI and NotI restriction sites"
     regulatory      2182..2419
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal; enhance RNA 
                     stability and translation"
     polyA_signal    2197..2318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2424..2678
                     /note="chicken beta-globin core insulator; shields 
                     transgene from chromosomal positional effects"
     misc_feature    2702..2714
                     /note="SphI and SbfI restriction sites to isolate transgene
                     construct"
     rep_origin      2799..3254
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3586..3690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3691..4551
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /note="ampicillin resistance"
                     /protein_id="AAM70477.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3691..4551
                     /gene="ampR"
                     /label=ampR
     rep_origin      4722..5310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"