LOCUS       Exported                4286 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRA, complete sequence.
ACCESSION   MG210576
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Wagner SG, Ziegler M, Kremling A, Pflueger-Grau K.
  TITLE     pTRA - a reporter system for the quantitative characterisation of 
            synthetic building blocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Wagner SG, Ziegler M, Kremling A, Pflueger-Grau K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2017) Systems Biotechnology, Technical University 
            Munich, Boltzmannstr. 15, Garching 85748, Germany
REFERENCE   3  (bases 1 to 4286)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Cloning vector pTRA"
                     /mol_type="other DNA"
                     /db_xref="taxon:2072507"
     regulatory      95..108
                     /regulatory_class="ribosome_binding_site"
     misc_feature    126..359
                     /label=F30-2xdBoccoli mRNA-tag
                     /note="F30-2xdBoccoli mRNA-tag"
     regulatory      371..384
                     /regulatory_class="ribosome_binding_site"
     CDS             387..692
                     /codon_start=1
                     /product="His-tagged-LacZ-alpha peptide"
                     /label=His-tagged-LacZ-alpha peptide
                     /protein_id="AUS45881.1"
                     /translation="MSSSHHHHHHHMGDPKLLLEVPHAISSSPGNSLAVVLQRRDWENP
                     GVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLIRLLTKPERKLSWLLPPLSNN"
     misc_feature    399..419
                     /label=Region: histidine-tag
                     /note="Region: histidine-tag"
     CDS             399..419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    420..689
                     /label=Region: LacZ-alpha peptide
                     /note="Region: LacZ-alpha peptide"
     CDS             complement(463..480)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     misc_feature    699..716
                     /label=his-tag
                     /note="his-tag"
     CDS             699..716
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    735..968
                     /function="F30-2xdBroccoli mRNA-tag"
     regulatory      990..1093
                     /regulatory_class="terminator"
                     /note="T0"
     terminator      993..1087
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1118..1146
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     gene            1335..1869
                     /gene="acc3"
                     /label=acc3
     CDS             1335..1868
                     /codon_start=1
                     /gene="acc3"
                     /product="gentamicin-3-acetyltransferase"
                     /label=acc3
                     /protein_id="AUS45882.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            1944..2187
     oriT            2079..2187
                     /note="incP origin of transfer"
     rep_origin      2281..2869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2923..3765)
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /protein_id="AUS45883.1"
                     /translation="MSNVASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVT
                     DEVMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGW
                     VQTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD
                     SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKV
                     THLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     gene            complement(2923..3765)
                     /gene="rep"
                     /label=rep
     CDS             complement(2923..3756)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3770..4121
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     regulatory      4181..4285
                     /regulatory_class="terminator"
                     /note="T1"
     terminator      4194..4280
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"