LOCUS       Exported                7404 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  AAV expression vector pTR-UF50-BC, complete sequence.
ACCESSION   KF926476
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Marsic D, Govindasamy L, Currlin S, Markusic DM, Tseng YS, Herzog 
            RW, Agbandje-McKenna M, Zolotukhin S.
  TITLE     Vector design Tour de Force: integrating combinatorial and rational 
            approaches to derive novel adeno-associated virus variants
  JOURNAL   Mol. Ther. 22 (11), 1900-1909 (2014)
  PUBMED    25048217
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Zolotukhin S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2013) Pediatrics, University of Florida, 2033 
            Mowry Rd, CGRC 235, Gainesville, FL 32610, USA
REFERENCE   3  (bases 1 to 7404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="AAV expression vector pTR-UF50-BC"
                     /mol_type="other DNA"
                     /db_xref="taxon:1463588"
     repeat_region   1..151
                     /rpt_family="AAV2 ITR"
     regulatory      203..2076
                     /regulatory_class="promoter"
                     /note="CBA promoter"
     enhancer        345..724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1003
                     /label=chicken beta-actin promoter
     intron          1004..2022
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     regulatory      2078..2087
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2084..4528
                     /codon_start=1
                     /product="luciferase-mApple fusion protein"
                     /label=luciferase-mApple fusion protein
                     /protein_id="AHJ81042.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAVRAKRAPVKQTLNFDLLKLAGDVESNP
                     GPMVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGG
                     PLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDS
                     SLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKSEIKKRLKLKDG
                     GHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAEGRHSTGGMDEL
                     YK"
     misc_feature    3734..3745
                     /label=Region: Furin cleavage sequence
                     /note="Region: Furin cleavage sequence"
     misc_feature    3746..3817
                     /label=Region: 2A
                     /note="Region: 2A"
     CDS             3752..3817
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3818..4528
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3818..4525
                     /label=Region: mApple
                     /note="Region: mApple"
     misc_feature    4535..4540
                     /label=Barcode
                     /note="Barcode"
     polyA_signal    4559..4680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4729..4874
                     /rpt_family="AAV2 ITR"
     CDS             complement(5875..6735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6736..6840)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6867..7295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"