LOCUS       Exported                5455 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  E. coli-T. kodakarensis shuttle vector pTPTK2, complete sequence.
ACCESSION   MG920816
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
  PUBMED    29497842
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 5455)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="E. coli-T. kodakarensis shuttle vector pTPTK2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2126327"
     rep_origin      1..845
                     /label=P15A_ORI
                     /note="P15A_ORI"
     rep_origin      complement(268..813)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             1253..2554
                     /codon_start=1
                     /product="TrpE"
                     /label=TrpE
                     /protein_id="AVP12437.1"
                     /translation="MPLKKLKPVDPLKLYSALRDFGMPFMLRSAEKDSRKARFTYISAE
                     PEFVVEVGEGTEIDGERVSDERNPLRALKGLMGERVEGRRFMGGFVGYVSYDSVHSIIG
                     GKIEEPSVFGYYPWTFIYDHSTGALSFFYLREAPFDPEALVERARREESRLEDGGSEVI
                     STDAGMEEFVEIVRAGKEYIYSGDVFQVVLSREYRVRTDLDALEIYKRLVELNPSPYTF
                     ILEFEKTVVGASPETMGSVEGRTFKINPIAGTAPRGRTGEEDRELEKALLSDEKERAEH
                     VMLVDLARNDVRRVSKPGSVRLTRFFDVLKYSHVQHIESEVVGELDEGKNAFDAMEAAF
                     PAGTLTGAPKIRAMEIIDELERSRRKVYGGAVGYFSLTGDADMAIAIRMAEIEGRKASV
                     RAGAGIVADSVPEKEFFETENKMRAVLKALGVRE"
     CDS             2426..2437
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2582..2716)
                     /codon_start=1
                     /product="transmembrane domain protein"
                     /label=transmembrane domain protein
                     /protein_id="AVP12436.1"
                     /translation="MSKKLDVKVKVNIYVNVHIDIKVAISIAVTIAVIALLKVLLNIR"
     CDS             2819..2968
                     /codon_start=1
                     /product="SpoVT/AbrB-like transcriptional regulator"
                     /label=SpoVT/AbrB-like transcriptional regulator
                     /note="swapped-hairpin fold CDS"
                     /protein_id="AVP12435.1"
                     /translation="MQDVRKMFRSGSSYVVAFPPSVIQKTKFRHQRTVRVRIYDDKIVI
                     VPLW"
     CDS             3631..4389
                     /codon_start=1
                     /product="RepTP2"
                     /label=RepTP2
                     /protein_id="AVP12434.1"
                     /translation="MTLMCLWFHTRTRFTSRAMLVQKLERYDSLFKVASARHTDAVFLT
                     LTTDPSRFSNLYEANRQFSHSFNRFMSRLRGYFARRGQHLEYIAVYEFTKSGLLHAHVI
                     IFGVRYLLPVRVISRWWSEAGQGRVVYIYRLRNVDGRWVWARRRPRDVRAGEGAEDYLK
                     KYLRKALRLASTLDTSDVKKVLPLALYWAFNKRFFTYSRSLLPSSRRTAEAVVSEYLWV
                     GTYRLEDLPDWVFWLPHRVYSPPSRGPPPT"
     CDS             complement(4435..5094)
                     /codon_start=1
                     /product="chloramphenicol resistance protein"
                     /label=chloramphenicol resistance protein
                     /protein_id="AVP12433.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5095..5197)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"