LOCUS       Exported                9026 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  E. coli-T. kodakarensis shuttle vector pTNTrpE, complete sequence.
ACCESSION   MG920814
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
  PUBMED    29497842
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 9026)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="E. coli-T. kodakarensis shuttle vector pTNTrpE"
                     /mol_type="other DNA"
                     /db_xref="taxon:2126325"
     rep_origin      1..620
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    893..914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        929..959
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1586..2236
                     /codon_start=1
                     /product="p24"
                     /label=p24
                     /protein_id="AVP12426.1"
                     /translation="MESGENTPYKKFICLHCGHVFDSDRDAPKCPNCGRRKVIPRETLG
                     ESLRKHKDKLKELGVLPEPDPEPQETEVGKAEVETAGQKDPETSKPEPIQVEQKVEELK
                     PESDQEPQNSESVDSPDDVPESDKLLAKYLEELEDVPAKPKPKKLRPKKSKRSITVPAG
                     PSFLEVLIIIGAIVLIWKWLSSRSSKSDDESNVPRPSETYYQIQRNLGHHVLG"
     CDS             2771..4696
                     /codon_start=1
                     /product="Rep74"
                     /label=Rep74
                     /protein_id="AVP12425.1"
                     /translation="MRLSNSSINSLLSSGSCDVVGSSPSGSPFLPQSTGSTQKTLSVYL
                     DISPTNEKGEPSVRGPTIRVSSHPLPAYAYYRSATGHLERQKFESQGRVKIDIYKLRKK
                     LFWIRRRLERVDLTDEERSSLEAERDRLVSLTEKLLRKIYSGSALYGDRFVIDVPVAYS
                     QLCKALGINPSDVGVSLFVRSAVMDLEFDDSSRSALKISYVSRLFAGKYHPAKGISKGF
                     KEARRVVRDLLVLQEFLDGSLLSYHKGDYVTSNSLLPMRFFVLTLPEEISYYIWSKLRE
                     GDDSALKLFKKISSQAIRDFLFYLAQKEGIPINKSYFVPGFLQNIHPTGDRDPFKPHFH
                     AHFSVVFVVYDKSSHTWYRLNPVLDEADLEKLREIWKALVVEAFSEILSGDTLTKDFNV
                     WVGDRYYSLPHDYVGVLFEIKYNARKMFVNYSSYYERNSFSDDFDRSFVSFIFDYKNRT
                     ERYGFLTNIKRYLSRLSISAVKKRLEELRELLDRIEADLLVVDSGRFPLLYQSLLDKRE
                     AVQSEISRLESVLNNPDDAFRVLYDEISRDVESMLSPRTVKENRIVSLLESLHGKRVVG
                     LSVEYIRYLTRDGEEVLDVPLHKFLEARRSVVLLSDRHKTVEFMLWWDPFWEDPPDVLE
                     LKIPNS"
     CDS             4859..6160
                     /codon_start=1
                     /product="TrpE"
                     /label=TrpE
                     /protein_id="AVP12424.1"
                     /translation="MPLKKLKPVDPLKLYSALRDFGMPFMLRSAEKDSRKARFTYISAE
                     PEFVVEVGEGTEIDGERVSDERNPLRALKGLMGERVEGRRFMGGFVGYVSYDSVHSIIG
                     GKIEEPSVFGYYPWTFIYDHSTGALSFFYLREAPFDPEALVERARREESRLEDGGSEVI
                     STDAGMEEFVEIVRAGKEYIYSGDVFQVVLSREYRVRTDLDALEIYKRLVELNPSPYTF
                     ILEFEKTVVGASPETMGSVEGRTFKINPIAGTAPRGRTGEEDRELEKALLSDEKERAEH
                     VMLVDLARNDVRRVSKPGSVRLTRFFDVLKYSHVQHIESEVVGELDEGKNAFDAMEAAF
                     PAGTLTGAPKIRAMEIIDELERSRRKVYGGAVGYFSLTGDADMAIAIRMAEIEGRKASV
                     RAGAGIVADSVPEKEFFETENKMRAVLKALGVRE"
     CDS             6032..6043
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(6246..6264)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6271..6287)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6428..6856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      6445..6751
                     /label=f1_origin
                     /note="f1_origin"
     CDS             7200..7994
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /protein_id="AVP12423.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8012..8872
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AVP12422.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"