LOCUS       Exported                3850 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTn7xG, complete sequence.
ACCESSION   KF813058
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Bruckbauer ST.
  TITLE     Tn5/7-lux: A versatile tool for the identification and capture of 
            promoters in Gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Bruckbauer ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2013) Microbiology, Immunology and Pathology, 
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO 
            80523, USA
REFERENCE   3  (bases 1 to 3850)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Cloning vector pTn7xG"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label=pTn5/7luxG4
                     /note="pTn5/7luxG4"
                     /db_xref="taxon:1454555"
     misc_feature    58..105
                     /label=FRT site
                     /note="FRT site"
     protein_bind    complement(58..105)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        146..174
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             363..896
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamicin acetyl transferase"
                     /label=aacC1
                     /note="AacC1"
                     /protein_id="AHH35068.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            363..896
                     /gene="aacC1"
                     /label=aacC1
     misc_feature    1026..1073
                     /label=FRT site
                     /note="FRT site"
     protein_bind    complement(1026..1073)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      1114..1211
                     /regulatory_class="terminator"
                     /note="phage lambda"
     terminator      1119..1205
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      1308..1402
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB operon"
     terminator      1308..1402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1430..1446)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1451..1650
                     /note="Tn7R; Tn7 right end"
     oriT            2249..2510
     oriT            2395..2503
                     /note="incP origin of transfer"
     rep_origin      2534..2915
                     /label=R6K
                     /note="R6K"
     rep_origin      2554..2910
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     repeat_region   3126..3291
                     /note="Tn7L; Tn7 left end"
     mobile_element  3126..3291
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    3322..3346
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3377..3842
                     /regulatory_class="promoter"
                     /note="Pseudomonas aeruginosa PA4974 gene"