LOCUS       Exported               11278 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTM117, complete sequence.
ACCESSION   EF540942
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Carpenter BM, McDaniel TK, Whitmire JM, Gancz H, Guidotti S, Censini
            S, Merrell DS.
  TITLE     Expanding the Helicobacter pylori Genetic Toolbox: Modification of 
            an Endogenous Plasmid for Use as a Transcriptional Reporter and 
            Complementation Vector
  JOURNAL   Appl. Environ. Microbiol. 73 (23), 7506-7514 (2007)
  PUBMED    17921278
REFERENCE   2  (bases 1 to 11278)
  AUTHORS   Carpenter BM, McDaniel TK, Whitmire JM, Gancz H, Guidotti S, Censini
            S, Covacci A, Merrell DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2007) Microbiology and Immunology, Uniformed 
            Services University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
REFERENCE   3  (bases 1 to 11278)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Cloning vector pTM117"
                     /lab_host="Helicobacter pylori"
                     /mol_type="other DNA"
                     /db_xref="taxon:441598"
     CDS             complement(16..738)
                     /codon_start=1
                     /gene="r2"
                     /product="putative cAMP-induced filamentation protein"
                     /label=r2
                     /note="R2"
                     /protein_id="ABQ18301.1"
                     /translation="MKRQLHLSKLIEKLNHERKNAIKNGIYHLIQIKFSYNSNRIEGSG
                     LTYEQTAHIFDKSVLITEKNANIKLDDIFETINHFECVNYLLESYQESLSLEYFKTLHK
                     ILKKNCSDEVIGGFKKRPNFVGNSATTRPQLVESELTNLVKNYQRNLEVSLENIIDFHV
                     AFEKIHPFSDGNGRVGRLVMFKECLKNNIMPFIIENEHKAFYYRGVKEYHNTKGYLKDT
                     ILQSQDNFNEMVSYFFSK"
     gene            complement(16..738)
                     /gene="r2"
                     /label=r2
     CDS             complement(735..2339)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA replicase"
                     /label=repA
                     /note="RepA"
                     /protein_id="ABQ18302.1"
                     /translation="MEFDQLESQRSDLQKVLKELDTLPKTPQIELQKQEIQDRINKITD
                     TIIKELLSKHEIKKEELEPTLKEKPTPTKVPQTTPTPCKDLVVSTPKDKTYTTYHNNTN
                     KVNLGKLSEREANLLFAIFQMLKDQGNTLIRFEPQDLKRMLNIDISNERLSEVVIKLWD
                     SIKTADFWKISETETSIIQENYMLFSRCKIELNKPSKDLKYLEIQLNDNYQYLLNNLGM
                     GQYTSFNLLEFQRVRGKYAKTLYRLLKQYKSTGILSVEWGQFRELLDIPKDYDMRSIDN
                     FVLKIALKELHKIYPFEHLSYKKERRNSHDRRKVTHIDFYFEQLPKGETKKQKQADKQR
                     AKRDIKLIAWDIHKEIAKRNAKDTMEARFLELKTLIGYQFRHNDSGIVLQIDNTAFEKN
                     QMFLHVSYPKSKHNPQKFLVSNKTFALELLYINGYSLKKDNLLEEIDPPKIHPITNEPI
                     KEFAQYIGKTINITNFNVDQCPKGINNYLKITRIVKLNDNRICISVQDVDKPEKLLKPF
                     IAKDEKHLNNWFKKHYR"
     gene            complement(735..2339)
                     /gene="repA"
                     /label=repA
     CDS             1104..1490
                     /codon_start=1
                     /gene="f3"
                     /product="hypothetical protein"
                     /label=f3
                     /note="F3"
                     /protein_id="ABQ18303.1"
                     /translation="MLAFRVRNMQKHLILFKSGVVNLQNDPTVIVPELIADQSFQFKKP
                     SFHSVFGVSFSDFFVNIPCYELDVPFSALLVGLFLFLGFAFGQLLKVEINVRYLAPVVA
                     VSAFFFVTQVFKRVDFMEFFESDF"
     gene            1104..1490
                     /gene="f3"
                     /label=f3
     rep_origin      2666..2766
                     /label=from pHP666
                     /note="from pHP666"
     CDS             3420..4832
                     /codon_start=1
                     /gene="f4"
                     /product="putative replicase"
                     /label=f4
                     /note="F4"
                     /protein_id="ABQ18304.1"
                     /translation="MLKDYWECFNFLTYNDYEEWSNGEDFYSFIFPNCESKGEINKDFS
                     KPNAVFLYKDLKTTLNDTDKPALKRRIMLKDTWGDDYIDFVLENDLTLCSGLSYRGRHN
                     DLAHAQQMNALIFDLDGVGLKEITAFFEVIKYCEKNEPNKYFWPIPRPTFVVLSGVGLH
                     VYYVFDKPIDLFPNIKLQLKAYKYALTFNIWRYKETSKEKETQYQSINQSFRMVGSINE
                     KHGNKIIAFKTGDRVSLEYMNQYVKEDKRVDILKRFRPSKHTLAEAKLKFPEWYEKVII
                     QGQTSERKKWRIKRDVYEWWKKRCKEPKGGHRYFFMMCMAIYAYKCDVSKQELEKDMLE
                     VFEKLKDIPHTNPLTKRDVYSALESYDKGLACFKIKDIEILTALRIDRNRRNYRQQYLH
                     LKGIRALQQAINPTWRQGNGRPKNSGIKQSLIQEWRIKNPQGKKIDCHKELGLSRPTID
                     KWWNSKQLAMTT"
     gene            3420..4832
                     /gene="f4"
                     /label=f4
     CDS             complement(4806..5072)
                     /codon_start=1
                     /gene="r3"
                     /product="hypothetical protein"
                     /label=r3
                     /note="R3"
                     /protein_id="ABQ18305.1"
                     /translation="MLDIEYHNIFRKDYKKYLKNGFDSKLLDEVVLELRQQKPLAQKHK
                     DHILKGEWYPCRECHIRPDVLLVYIVKNNTLWLLRLGSHSELF"
     gene            complement(4806..5072)
                     /gene="r3"
                     /label=r3
     CDS             complement(5059..5337)
                     /codon_start=1
                     /gene="r4"
                     /product="hypothetical protein"
                     /label=r4
                     /note="R4"
                     /protein_id="ABQ18306.1"
                     /translation="MLKDVEVGVKFYKELGKLEKQLAKYQSKVLEIKKQMREIKKQYSQ
                     AKKDEKKAKNLETLQAIKDVDSGANFTKIDSLDELTNELKREIHAGY"
     gene            complement(5059..5337)
                     /gene="r4"
                     /label=r4
     CDS             5539..5883
                     /codon_start=1
                     /gene="mobC"
                     /product="MobC mobilization protein"
                     /label=mobC
                     /protein_id="ABQ18307.1"
                     /translation="MPKLSDNKNITKRLRLNQTEWQIIQTQMQEKNLNFSQLVLNSLLT
                     QNSQTPTRSKKQKAIANKELVIELAKWGNNLNQIAKCLNTNKGAWDRLGLEQLIEISYQ
                     LEQIRSKYVG"
     gene            5539..5883
                     /gene="mobC"
                     /label=mobC
     CDS             5873..7888
                     /codon_start=1
                     /gene="mobA"
                     /product="MobA mobilization protein"
                     /label=mobA
                     /note="MobA"
                     /protein_id="ABQ18308.1"
                     /translation="MLVKFWGINQGGGDGDGSVNYLLNERVKQGTAKVLKGDANLTKSL
                     LLSLTQKHKACVGCLSFEESNIDESLKYELMESFENALLTQEMQGRYNILWVEHTDKGR
                     LELNFVIPKIDLEKQKAFNPYYHKVDLKRIDTWKDCINLKHNFTNPKDLEKQQISNYHN
                     TKTPQGKAIFKNYKELDNYLYNAVTQGLLNSRAEIIELLQASNYEITRQGKDYISVKLP
                     NQQKAKRLKGIIYDEQFTEQRLIEQELREYSQNKDTSRERDTREISTAPNTTPRATQSL
                     IQELRDLEQQLHRHIEYKQRYYKKLHQSTSKDPREPKEQLPRVRDSQQRTQQNHANETT
                     RARETNPQEPSQEILTNAPNFHHRSSHHGGNISGGILSHHSTKQGQGIRAFTERSLSVK
                     TRNRPTKQRQSTENLQPNADQEIRGKNAGRILKQATERFNQRSRELTERERDLAQRKEQ
                     RNREFRERQKRAINQARESYSRISNAKQRLLEIRGRNLELERELRNAIPNAQHIISQIS
                     SFRESRESRIKSINANLRTERNHAQKRFTEHVRADFTTRITERSNQRARDFTSSIQRIE
                     NTIRDFRNNAQARVRTILHNLYARTRDAINTIKDIYRNAKERIRDFFQRADERLSKRME
                     QEARELHTKTTQLDRKIREHNQEIDDERSYSCGYGR"
     gene            5873..7888
                     /gene="mobA"
                     /label=mobA
     CDS             7949..8083
                     /codon_start=1
                     /gene="f2"
                     /product="hypothetical protein"
                     /label=f2
                     /note="F2"
                     /protein_id="ABQ18309.1"
                     /translation="MKEYKIFISHSWKYDNHYANLQDALKDLAEEIQPKVYEYGFLRE"
     gene            7949..8083
                     /gene="f2"
                     /label=f2
     CDS             complement(8128..8922)
                     /codon_start=1
                     /gene="aphA3"
                     /product="AphA3"
                     /label=aphA3
                     /note="from Campylobacter coli"
                     /protein_id="ABQ18310.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(8128..8922)
                     /gene="aphA3"
                     /label=aphA3
     misc_feature    9086..9110
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             9131..9847
                     /codon_start=1
                     /gene="gfp"
                     /product="mut3 GFP derivative"
                     /label=gfp
                     /note="GFP (mut3)"
                     /protein_id="ABQ18311.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            9131..9847
                     /gene="gfp"
                     /label=gfp
     CDS             10022..10213
                     /codon_start=1
                     /gene="rop"
                     /product="Rop"
                     /label=rop
                     /note="modulator of copy number"
                     /protein_id="ABQ18312.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     gene            10022..10213
                     /gene="rop"
                     /label=rop
     misc_feature    10315..10455
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      10639..11239
                     /label=from Escherichia coli
                     /note="from Escherichia coli"
     rep_origin      complement(10641..11229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"