LOCUS       Exported                4143 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway Entry vector pTK185, complete sequence.
ACCESSION   FJ410921
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Rozwadowski K, Yang W, Kagale S.
  TITLE     Homologous recombination-mediated cloning and manipulation of 
            genomic DNA regions using Gateway and recombineering systems
  JOURNAL   BMC Biotechnol. 8, 88 (2008)
  PUBMED    19014699
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Rozwadowski KL, Yang W, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2008) Molecular Genetics, Agriculture and 
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 
            0X2, Canada
REFERENCE   3  (bases 1 to 4143)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Gateway Entry vector pTK185"
                     /mol_type="other DNA"
                     /db_xref="taxon:587429"
     misc_recomb     67..166
                     /label=attL1
                     /note="attL1"
     protein_bind    67..166
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             200..2008
                     /codon_start=1
                     /gene="GUS"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /protein_id="ACL35319.1"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            200..2008
                     /gene="GUS"
                     /label=GUS
     misc_recomb     2084..2183
                     /label=attL2
                     /note="attL2"
     protein_bind    complement(2084..2183)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2394..2768)
                     /codon_start=1
                     /gene="zeoR"
                     /product="ZeoR"
                     /label=zeoR
                     /protein_id="ACL35320.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            complement(2394..2768)
                     /gene="zeoR"
                     /label=zeoR
     promoter        complement(2787..2834)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      3112..3217
                     /label=pUC ori
                     /note="pUC ori"
     rep_origin      3202..3790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      3955..4041
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      3958..4001
                     /regulatory_class="terminator"
                     /note="rrnB_T1"
     regulatory      4133..17
                     /regulatory_class="terminator"
                     /note="rrnB_T2"
     terminator      4133..17
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"