LOCUS       Exported                8346 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTip-NH2.2 DNA, complete sequence.
ACCESSION   AB094129
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Nakashima N, Tamura T.
  TITLE     A novel system for expressing recombinant proteins over a wide 
            temperature range from 4 to 35 degrees C
  JOURNAL   Biotechnol. Bioeng. 86 (2), 136-148 (2004)
  PUBMED    15052633
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Nakashima N, Tamura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2002) Nobutaka Nakashima, National Institute of 
            Advanced Industrial Science and Technology; Tsukisamu-Higashi 
            2-17-2-1, Sapporo, Toyohira-ku 062-8517, Japan 
            (E-mail:n-nakashima@aist.go.jp, Tel:81-11-857-8937)
REFERENCE   3  (bases 1 to 8346)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Expression vector pTip-NH2.2"
                     /mol_type="other DNA"
                     /db_xref="taxon:211481"
     CDS             170..979
                     /codon_start=1
                     /gene="tsr"
                     /product="thiostrepton resistance protein"
                     /label=tsr
                     /protein_id="BAD11268.1"
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     gene            170..979
                     /gene="tsr"
                     /label=tsr
     CDS             1541..2716
                     /codon_start=1
                     /gene="Chlr"
                     /product="chloramphenicol resistance protein"
                     /label=Chlr
                     /protein_id="BAD11269.1"
                     /translation="VPFAIYVLGLAVFAQGTSEFMLSGLIPDMARDLGVSVPAAGLLTS
                     AFAVGMIIGAPLMAIASMRWPRRRALLTFLITFMLVHVIGALTSSFEVLLVTRIVGALA
                     NAGFLAVALGAAMAMVPADMKGRATSVLLGGVTIACVAGVPGGAFLGEMWGWRAAFWAV
                     VVISAPAVVAIMFATPAEPLAESTPNAKRELSSLRSRKLQLMLVLGALINGATFCSFTY
                     MAPTLTDISGFDSRWIPLLLGLFGLGSFIGVSVGGRLADTRPFQLLAVGSAALLTGWIV
                     FALTASHPAVTLVMLFVQGALSFAVGSTLISQVLYAADAAPTLGGSFATAAFNVGAALG
                     PALGGLAIGMGLSYRAPLWTSAALVTLAIVIGAATLSLWRRPASVHESVPA"
     gene            1541..2716
                     /gene="Chlr"
                     /label=Chlr
     regulatory      2905..3080
                     /regulatory_class="promoter"
                     /gene="ThcA"
     gene            2905..3080
                     /gene="ThcA"
                     /label=ThcA
     CDS             3081..3842
                     /codon_start=1
                     /gene="TipA"
                     /label=TipA
                     /protein_id="BAD11270.1"
                     /translation="MSYSVGQVAGFAGVTVRTLHHYDDIGLLVPSERSHAGHRRYSDAD
                     LDRLQQILFYRELGFPLDEVAALLDDPAADPRAHLRRQHELLSARIGKLQKMAAAVEQA
                     MEARSMGINLTPEEKFEVFGDFDPDQYEEEVRERWGNTDAYRQSKEKTASYTKEDWQRI
                     QDEADELTRRFVALMDAGEPADSEGAMDAAEDHRQGIARNHYDCGYEMHTCLGEMYVSD
                     ERFTRNIDAAKPGLAAYMRDAILANAVRHTP"
     gene            3081..3842
                     /gene="TipA"
                     /label=TipA
     promoter        4020..4124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4125..4985
                     /codon_start=1
                     /gene="Ampr"
                     /product="ampicillin resistance protein"
                     /label=Ampr
                     /protein_id="BAD11271.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4125..4985
                     /gene="Ampr"
                     /label=Ampr
     rep_origin      4986..5988
                     /label=ColE1
                     /note="ColE1"
     rep_origin      5156..5744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(5995..6175)
                     /regulatory_class="terminator"
                     /gene="ThcA"
     gene            complement(5995..6175)
                     /gene="ThcA"
                     /label=ThcA
     CDS             complement(6240..6257)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     gene            complement(6264..6406)
                     /gene="TipA"
                     /label=TipA
     regulatory      complement(6264..6282)
                     /regulatory_class="ribosome_binding_site"
                     /gene="TipA"
     regulatory      complement(6283..6406)
                     /regulatory_class="promoter"
                     /gene="TipA"
     CDS             6945..7832
                     /codon_start=1
                     /gene="RepA"
                     /label=RepA
                     /protein_id="BAD11272.1"
                     /translation="MKSATGGVLSDRQWADAVLLPHRPFATNNLQRGQYRMSRDDALAM
                     RYVEHSPHALLGSIVIDCDHVDAAMRAFEQPSDHPAPNWVAQSPSGRAHIGWWLGPNHV
                     CRTDSARLTPLRYAHRIETGLKISVGGDFAYGGQLTKNPIHPDWETIYGPATPYTLRQL
                     ATIHTPRQMPRRPDRAVGLGRNVTMFDATRRWAYPQWWQHRNGTGRDWDHLVLQHCHAV
                     NTEFTTPLPFTEVRATAQSISKWIWRNFTEEQYRARQAHLGQKGGKATTLAKQEAVRNN
                     ARKYDEHTMREAII"
     gene            6945..7832
                     /gene="RepA"
                     /label=RepA
     CDS             7832..8116
                     /codon_start=1
                     /gene="RepB"
                     /label=RepB
                     /protein_id="BAD11273.1"
                     /translation="MGGAKNPVRRKMTAAAAAEKFGASTRTIQRLFAEPRDDYLGRAKA
                     RRDKAVELRKQGLKYREIAEAMELSTGIVGRLLHDARRHGEISAEDLSA"
     gene            7832..8116
                     /gene="RepB"
                     /label=RepB