LOCUS       Exported                8184 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTip-LCH2 DNA, complete sequence.
ACCESSION   AB094126
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Nakashima N, Tamura T.
  TITLE     A novel system for expressing recombinant proteins over a wide 
            temperature range from 4 to 35 degrees C
  JOURNAL   Biotechnol. Bioeng. 86 (2), 136-148 (2004)
  PUBMED    15052633
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Nakashima N, Tamura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2002) Nobutaka Nakashima, National Institute of 
            Advanced Industrial Science and Technology; Tsukisamu-Higashi 
            2-17-2-1, Sapporo, Toyohira-ku 062-8517, Japan 
            (E-mail:n-nakashima@aist.go.jp, Tel:81-11-857-8937)
REFERENCE   3  (bases 1 to 8184)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Expression vector pTip-LCH2"
                     /mol_type="other DNA"
                     /db_xref="taxon:211478"
     CDS             170..979
                     /codon_start=1
                     /gene="tsr"
                     /product="thiostrepton resistance protein"
                     /label=tsr
                     /protein_id="BAD11250.1"
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     gene            170..979
                     /gene="tsr"
                     /label=tsr
     regulatory      1145..1324
                     /regulatory_class="promoter"
                     /gene="Tuf1"
     gene            1145..1324
                     /gene="Tuf1"
                     /label=Tuf1
     CDS             1325..2515
                     /codon_start=1
                     /gene="Tetr"
                     /product="tetracycline resistance protein"
                     /label=Tetr
                     /protein_id="BAD11251.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            1325..2515
                     /gene="Tetr"
                     /label=Tetr
     regulatory      2728..2903
                     /regulatory_class="promoter"
                     /gene="ThcA"
     gene            2728..2903
                     /gene="ThcA"
                     /label=ThcA
     CDS             2904..3665
                     /codon_start=1
                     /gene="TipA"
                     /label=TipA
                     /protein_id="BAD11252.1"
                     /translation="MSYSVGQVAGFAGVTVRTLHHYDDIGLLVPSERSHAGHRRYSDAD
                     LDRLQQILFYRELGFPLDEVAALLDDPAADPRAHLRRQHELLSARIGKLQKMAAAVEQA
                     MEARSMGINLTPEEKFEVFGDFDPDQYEEEVRERWGNTDAYRQSKEKTASYTKEDWQRI
                     QDEADELTRRFVALMDAGEPADSEGAMDAAEDHRQGIARNHYDCGYEMHTCLGEMYVSD
                     ERFTRNIDAAKPGLAAYMRDAILANAVRHTP"
     gene            2904..3665
                     /gene="TipA"
                     /label=TipA
     promoter        3843..3947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3948..4808
                     /codon_start=1
                     /gene="Ampr"
                     /product="ampicillin resistance protein"
                     /label=Ampr
                     /protein_id="BAD11253.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3948..4808
                     /gene="Ampr"
                     /label=Ampr
     rep_origin      4809..5811
                     /label=ColE1
                     /note="ColE1"
     rep_origin      4979..5567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(5818..5998)
                     /regulatory_class="terminator"
                     /gene="ThcA"
     gene            complement(5818..5998)
                     /gene="ThcA"
                     /label=ThcA
     CDS             complement(6012..6029)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     gene            complement(6078..6244)
                     /gene="TipA"
                     /label=TipA
     regulatory      complement(6078..6120)
                     /regulatory_class="ribosome_binding_site"
                     /gene="TipA"
     RBS             6085..6107
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      complement(6121..6244)
                     /regulatory_class="promoter"
                     /gene="TipA"
     CDS             6783..7670
                     /codon_start=1
                     /gene="RepA"
                     /label=RepA
                     /protein_id="BAD11254.1"
                     /translation="MKSATGGVLSDRQWADAVLLPHRPFATNNLQRGQYRMSRDDALAM
                     RYVEHSPHALLGSIVIDCDHVDAAMRAFEQPSDHPAPNWVAQSPSGRAHIGWWLGPNHV
                     CRTDSARLTPLRYAHRIETGLKISVGGDFAYGGQLTKNPIHPDWETIYGPATPYTLRQL
                     ATIHTPRQMPRRPDRAVGLGRNVTMFDATRRWAYPQWWQHRNGTGRDWDHLVLQHCHAV
                     NTEFTTPLPFTEVRATAQSISKWIWRNFTEEQYRARQAHLGQKGGKATTLAKQEAVRNN
                     ARKYDEHTMREAII"
     gene            6783..7670
                     /gene="RepA"
                     /label=RepA
     CDS             7670..7954
                     /codon_start=1
                     /gene="RepB"
                     /label=RepB
                     /protein_id="BAD11255.1"
                     /translation="MGGAKNPVRRKMTAAAAAEKFGASTRTIQRLFAEPRDDYLGRAKA
                     RRDKAVELRKQGLKYREIAEAMELSTGIVGRLLHDARRHGEISAEDLSA"
     gene            7670..7954
                     /gene="RepB"
                     /label=RepB