LOCUS       Exported                3942 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Insect cell expression vector pTIE1, complete sequence.
ACCESSION   AY522575
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Crawford F, Huseby E, White J, Marrack P, Kappler JW.
  TITLE     Mimotopes for alloreactive and conventional T cells in a peptide-MHC
            display library
  JOURNAL   PLoS Biol. 2 (4), E90 (2004)
  PUBMED    15094798
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Kappler J.
  TITLE     pTIE1 - hr5/IE1 Based Insect Cell Expression Vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3942)
  AUTHORS   Kappler J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2004) Integrated Department of Immunology, 
            HHMI-National Jewish Research and Medical Center, 1400 Jackson St., 
            Denver, CO 80206, USA
REFERENCE   4  (bases 1 to 3942)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Insect cell expression vector pTIE1"
                     /mol_type="other DNA"
                     /db_xref="taxon:266783"
     misc_feature    1..259
                     /label=pTZ18R backbone
                     /note="pTZ18R backbone"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        236..254
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      290..801
                     /regulatory_class="enhancer"
                     /note="Baculovirus hr5 enhancer/OOR"
     enhancer        313..795
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     regulatory      832..1429
                     /regulatory_class="promoter"
                     /note="Baculovirus IE1 promoter"
     promoter        833..1424
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     misc_feature    1430..1530
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3UTR           1531..1779
                     /label=Baculovirus IE1 3 UTR
                     /note="Baculovirus IE1 3 UTR"
     misc_feature    1780..3942
                     /label=pTZ18R backbone
                     /note="pTZ18R backbone"
     promoter        2043..2147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2148..3008
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3179..3767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"