LOCUS       Exported                6279 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pTEX5501ts, complete sequence.
ACCESSION   DQ208937
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Nallapareddy SR, Singh KV, Murray BE.
  TITLE     Construction of Improved Temperature-Sensitive and Mobilizable 
            Vectors and Their Use for Constructing Mutations in the 
            Adhesin-Encoding acm Gene of Poorly Transformable Clinical 
            Enterococcus faecium Strains
  JOURNAL   Appl. Environ. Microbiol. 72 (1), 334-345 (2006)
  PUBMED    16391062
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Nallapareddy SR, Singh KV, Murray BE.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2005) Infectious Diseases, Univ. of Texas Med. 
            School at Houston, 6431 Fannin St, MSB 2.112, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 6279)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Shuttle vector pTEX5501ts"
                     /mol_type="other DNA"
                     /db_xref="taxon:352214"
     misc_feature    1..61
                     /note="multiple cloning site I; MCS-I"
     CDS             195..845
                     /codon_start=1
                     /gene="cat"
                     /product="CAT"
                     /label=cat
                     /note="chloramphenicol acetyltransferase; promoter 
                     (synthetic aad9) derived from pFW11"
                     /protein_id="ABB20800.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            195..845
                     /gene="cat"
                     /label=cat
     misc_feature    1007..1076
                     /note="multiple cloning site II; MCS-II"
     CDS             complement(1479..1490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1624..1833
                     /codon_start=1
                     /gene="orfB"
                     /product="OrfB"
                     /label=orfB
                     /note="portion of pWV01 based temperature-sensitive 
                     gram-positive replicon derived from pTV1-OK"
                     /protein_id="ABB20801.1"
                     /translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES
                     PTFSPTFFINLYLSSFIVVFMITK"
     gene            1624..1833
                     /gene="orfB"
                     /label=orfB
     CDS             1874..2035
                     /codon_start=1
                     /product="OrfC"
                     /label=OrfC
                     /note="portion of pWV01 based temperature-sensitive 
                     gram-positive replicon derived from pTV1-OK"
                     /protein_id="ABB20802.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     CDS             2102..2800
                     /codon_start=1
                     /gene="repA(ts)"
                     /product="RepA(ts)"
                     /label=repA(ts)
                     /note="portion of pWV01 based temperature-sensitive 
                     gram-positive replicon derived from pTV1-OK"
                     /protein_id="ABB20803.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDLYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            2102..2800
                     /gene="repA(ts)"
                     /label=repA(ts)
     CDS             2797..2946
                     /codon_start=1
                     /gene="orfD"
                     /product="OrfD"
                     /label=orfD
                     /note="portion of pWV01 based temperature-sensitive 
                     gram-positive replicon derived from pTV1-OK"
                     /protein_id="ABB20804.1"
                     /translation="MTNKEKELFAENEELKKEIKDLKERIERYREMEVELSTTIDLLRG
                     GIIE"
     gene            2797..2946
                     /gene="orfD"
                     /label=orfD
     CDS             3173..4078
                     /codon_start=1
                     /gene="aph(2)-Id"
                     /product="APH(2)-Id"
                     /label=aph(2)-Id
                     /note="aminoglycoside modifying enzyme; derived from 
                     Enterococcus casseliflavus in GenBank Accession Number 
                     AF016483"
                     /protein_id="ABB20805.1"
                     /translation="MRTYTFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEINRDFIF
                     KFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLT
                     PLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSR
                     ELKGPQMKIVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAA
                     ISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKE
                     YGYMDWYEEGLNEIRSIKIK"
     gene            3173..4078
                     /gene="aph(2)-Id"
                     /label=aph(2)-Id
     oriT            4172..4838
                     /note="oriT of Tn916 derived from pHS1; facilitates 
                     conjugative transfer from the transformable E. faecalis 
                     strain JH2Sm::Tn916 to E. faecium"
     rep_origin      5036..5634
                     /note="Escherichia coli OriR from plasmid pUC18; derived 
                     from pFW14"
     rep_origin      complement(5036..5624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"