LOCUS       Exported                5874 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTEX2, complete sequence.
ACCESSION   KY242357
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Meiers M, Laux A, Eichinger D, Sexauer A, Marx P, Bertram R, 
            Bruckner R.
  TITLE     A tetracycline inducible integrative expression system for 
            Streptococcus pneumoniae
  JOURNAL   FEMS Microbiol. Lett. (2017) In press
  PUBMED    28333198
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Bruckner R.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2016) Microbiology, University of Kaiserslautern, 
            Paul-Ehrlich Str. 24, Kaiserslautern, RP D-67663, Germany
REFERENCE   3  (bases 1 to 5874)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Cloning vector pTEX2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1972734"
     protein_bind    53..70
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     regulatory      complement(75..139)
                     /regulatory_class="promoter"
                     /note="inducible promoter Pxyl/tet"
     protein_bind    87..105
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             212..838
                     /codon_start=1
                     /gene="tetR"
                     /product="TetR"
                     /label=tetR
                     /note="tetracycline repressor"
                     /protein_id="ARB66473.1"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            212..838
                     /gene="tetR"
                     /label=tetR
     CDS             215..838
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     misc_recomb     910..1894
                     /label=integration site S. pneumoniae
                     /note="integration site S. pneumoniae"
     misc_feature    2036..2176
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2362..2950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3121..3981)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ARB66474.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3982..4086)
                     /gene="bla"
                     /label=AmpR promoter
     misc_recomb     4191..5252
                     /label=integration site S. pneumoniae
                     /note="integration site S. pneumoniae"
     CDS             complement(5300..5797)
                     /codon_start=1
                     /gene="tmp"
                     /product="dihydrofolate reductase"
                     /label=tmp
                     /note="trimethoprim resistant protein"
                     /protein_id="ARB66475.1"
                     /translation="MNPESVRIYLVAAMGANRVIGNGPDIPWKIPGEQKIFRRLTESKV
                     VVMGRKTFESIGKPLPNRHTVVLSRQAGYSAPGCAVVSTLSQAIAIAAEHGKELYVARG
                     AEVYALALPHANGVFLSEVHQTFEGDAFFPVLNAAEFEVVSSETIQGTITYTHSVYARR
                     NG"
     gene            complement(5300..5797)
                     /gene="tmp"
                     /label=tmp