LOCUS       Exported                6151 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pTET15, complete sequence.
ACCESSION   FJ859896
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Huber J, Donald RG, Lee SH, Jarantow LW, Salvatore MJ, Meng X, 
            Painter R, Onishi RH, Occi J, Dorso K, Young K, Park YW, Skwish S, 
            Szymonifka MJ, Waddell TS, Miesel L, Phillips JW, Roemer T.
  TITLE     Chemical genetic identification of peptidoglycan inhibitors 
            potentiating carbapenem activity against methicillin-resistant 
            Staphylococcus aureus
  JOURNAL   Chem. Biol. 16 (8), 837-848 (2009)
  PUBMED    19716474
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Trawick J, Haselbeck B.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2009) Infectious Diseases, Merck & Co., 126 E. 
            Lincoln Ave, Rahway, NJ 07065, USA
REFERENCE   3  (bases 1 to 6151)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Shuttle vector pTET15"
                     /lab_host="Staphylococcus aureus"
                     /mol_type="other DNA"
                     /note="designed for tet-regulated gene expression in 
                     Staphylococcus sp."
                     /db_xref="taxon:696860"
     protein_bind    210..231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        246..276
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    284..300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(349..972)
                     /codon_start=1
                     /gene="tetR"
                     /product="Tet repressor"
                     /label=tetR
                     /note="derived from transposon Tn10"
                     /protein_id="ACZ60058.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(349..972)
                     /gene="tetR"
                     /label=tetR
     protein_bind    997..1015
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     regulatory      1046..1107
                     /regulatory_class="promoter"
                     /note="hybrid xylose/tetracycline promoter"
     protein_bind    1072..1089
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     terminator      1324..1410
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1502..1529
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(1672..2532)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="confers ampicillin resistance in Escherichia coli"
                     /protein_id="ACZ60056.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2533..2637)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2662..3500
                     /label=for replication in Escherichia coli
                     /note="for replication in Escherichia coli"
     rep_origin      2857..3445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4242..4892)
                     /codon_start=1
                     /product="CAT"
                     /label=CAT
                     /note="chloramphenicol acetyl transferase; confers 
                     chloramphenicol resistance in Staphylococcus aureus"
                     /protein_id="ACZ60057.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     rep_origin      5049..5212
                     /note="for replication in Staphylococcus sp."