LOCUS       Exported                3052 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTD1(TM) DNA, complete sequence.
ACCESSION   AB194742
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Suzuki T, Ito M, Ezure T, Kobayashi S, Shikata M, Tanimizu K, 
            Nishimura O.
  TITLE     Performance of expression vector, pTD1, in insect cell-free 
            translation system
  JOURNAL   J. Biosci. Bioeng. 102 (1), 69-71 (2006)
  PUBMED    16952840
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Suzuki T, Shikata M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2004) Masamitsu Shikata, SHIMADZU CORPORATION, 
            Analytical & Measuring Instruments Division, Life Science 
            Laboratory; 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto, Kyoto 
            604-8511, Japan (E-mail:mshikata@shimadzu.co.jp, 
            URL:http://www.shimadzu.com, Tel:81-75-823-1351, Fax:81-75-823-1368)
REFERENCE   3  (bases 1 to 3052)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Expression vector pTD1(TM)"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /note="circular complete sequence; designed for in vitro 
                     translation system"
                     /db_xref="taxon:300847"
     misc_feature    join(1..237,813..3052)
                     /label=pUC19-derived sequence
                     /note="pUC19-derived sequence"
     regulatory      240..258
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    259..304
                     /label=MnNPV 5 polyhedrin leader sequence
                     /note="MnNPV 5 polyhedrin leader sequence"
     5UTR           259..304
                     /label=polyhedrin 5 UTR
                     /note="5 UTR from the Malacosoma neustria 
                     nucleopolyhedrovirus polyhedrin gene (Ezure et al., 2006)"
     misc_feature    305..342
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     misc_feature    358..731
                     /label=AcNPV polyhedrin gene 3 UTR
                     /note="AcNPV polyhedrin gene 3 UTR"
     3UTR           359..731
                     /label=polyhedrin 3 UTR
                     /note="3 UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     misc_feature    736..757
                     /label=synthetic poly(A)20 region
                     /note="synthetic poly(A)20 region"
     regulatory      758..805
                     /regulatory_class="terminator"
                     /note="T7 RNA polymerase terminator"
     terminator      758..805
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(831..847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    855..871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(879..909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    924..945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1233..1821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1992..2852)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="BAD77830.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2853..2957)
                     /gene="bla"
                     /label=AmpR promoter