LOCUS       Exported                3018 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Positive selection vector pTCS DNA, complete sequence.
ACCESSION   AB353144
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ohashi-Kunihiro S, Yohda M, Masaki H, Machida M.
  TITLE     A novel vector for positive selection of inserts harboring an open 
            reading frame by translational coupling
  JOURNAL   BioTechniques 43 (6), 751-752 (2007)
  PUBMED    18251250
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Ohashi S, Machida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2007) Contact:Sumiko Ohashi National Institute of 
            Advanced Industrial Science and Technology (AIST), Research 
            Institute for Cell Engineering; Central 6, 1-1, Higashi, Tsukuba, 
            Ibaraki 305-8566, Japan
REFERENCE   3  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Positive selection vector pTCS"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:457754"
     source          1..401
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
                     /db_xref="taxon:31851"
     source          749..3018
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
                     /db_xref="taxon:31851"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(410..667)
                     /codon_start=1
                     /gene="immE3"
                     /product="colicin E3 immunity protein"
                     /label=immE3
                     /protein_id="BAF99005.1"
                     /translation="MGLKLDLTWFDKSTEDFKGEEYSKDFGDDGSVMESLGVPFKDNVN
                     NGCFDVIAEWVPLLQPYFNHQIDISDNEYFVSFDYRDGDW"
     gene            complement(410..667)
                     /gene="immE3"
                     /label=immE3
     primer_bind     complement(797..813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    821..837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(845..875)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    890..911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1199..1787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1958..2818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2819..2923)
                     /gene="bla"
                     /label=AmpR promoter