LOCUS       Exported                3348 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-GA-ble-minus, complete sequence.
ACCESSION   JN596086
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3348)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Cloning vector pTcR-GA-ble-minus"
                     /mol_type="other DNA"
                     /db_xref="taxon:1133393"
     misc_feature    1..52
                     /label=MCS
                     /note="MCS"
     intron          complement(50..498)
                     /gene="actin"
     gene            complement(50..498)
                     /gene="actin"
                     /label=actin
                     /note="I actin"
     CDS             complement(499..873)
                     /codon_start=1
                     /gene="ble"
                     /product="ble"
                     /label=ble
                     /note="bleomycin resistance protein"
                     /protein_id="AEZ51459.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVENDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            complement(499..873)
                     /gene="ble"
                     /label=ble
     intron          complement(874..1386)
                     /gene="IgapdhI-II"
     gene            complement(874..1386)
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     misc_feature    1389..1444
                     /label=MCS
                     /note="MCS"
     rep_origin      complement(1568..2241)
                     /direction=LEFT
     rep_origin      complement(1627..2215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2386..3246)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /note="beta-lactamase"
                     /protein_id="AEZ51460.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2386..3246)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(3247..3)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(3247..3345)
                     /regulatory_class="promoter"
                     /gene="bla"
     gene            complement(3247..3345)
                     /gene="bla"
                     /label=bla