LOCUS       Exported                7750 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pTCH-PB, complete sequence.
ACCESSION   KF640085
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Shao M, Kumar S, Thomson JG.
  TITLE     Precise excision of plastid DNA by the large serine recombinase Bxb1
  JOURNAL   Plant Biotechnol. J. (2013) In press
  PUBMED    24261912
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Shao M, Kumar S, Thomson JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2013) Crop Improvement and Utilization, USDA, 800 
            Buchanan Street, Albany, CA 94710, USA
REFERENCE   3  (bases 1 to 7750)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Chloroplast transformation vector pTCH-PB"
                     /mol_type="other DNA"
                     /db_xref="taxon:1429095"
     misc_feature    1..824
                     /note="plastid sequence targeting insertions in the 
                     trnI-trnA intergenic region II; similar to INSD Accession 
                     Number Z00044"
     misc_feature    837..3200
                     /note="pUC118 vector sequence; similar to INSD Accession 
                     Number U07649"
     rep_origin      complement(1073..1661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1832..2692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2693..2797)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    3021..4438
                     /note="plastid sequence targeting insertions in the 
                     trnI-trnA intergenic region I; similar to INSD Accession 
                     Number Z00044"
     regulatory      4470..4586
                     /regulatory_class="promoter"
                     /note="plastid rRNA operon PEP promoter (Prrn)"
     5UTR           4591..4607
                     /note="psbA; similar to INSD Accession Number NC_001879"
     CDS             4610..5401
                     /codon_start=1
                     /gene="aadA"
                     /allele="aadA"
                     /product="aminoglycoside 3-adenylyltransferase"
                     /label=aadA
                     /note="confers the resistance to spectinomycin or 
                     streptomycin in bacteria and plants"
                     /protein_id="AHB17667.1"
                     /translation="MAEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            4610..5401
                     /gene="aadA"
                     /allele="aadA"
                     /label=aadA
                     /note="aminoglycoside 3-adenylyltransferase"
     protein_bind    5445..5478
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     5517..5622
                     /note="ParA resolution site; similar to INSD Accession 
                     Number M59825"
     misc_recomb     5634..5752
                     /note="CinH resolution site; similar to INSD Accession 
                     Number AF213017"
     misc_recomb     5792..5843
                     /note="Bxb1 attP52 recognition site; similar to INSD 
                     Accession Number JQ305140"
     misc_recomb     5904..5960
                     /note="phiC31 attP57 recognition site; similar to INSD 
                     Accession Number GU56446"
     regulatory      6017..6166
                     /regulatory_class="terminator"
                     /note="rps16 3UTR; similar to INSD Accession Number 
                     NC_001879"
     regulatory      6176..6278
                     /regulatory_class="promoter"
                     /note="psbA; similar to INSD Accession Number NC_001879"
     5UTR           6279..6363
                     /note="psbA; similar to INSD Accession Number NC_001879"
     CDS             6372..7211
                     /codon_start=1
                     /gene="sulI"
                     /product="dihydropteroate synthase"
                     /label=sulI
                     /note="stuffer region; stroma targeting domain removed; 
                     sulfonamide resistance protein; confers resistance to 
                     sulfadiazine in bacteria and plants"
                     /protein_id="AHB17668.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG
                     PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ
                     GFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSALRRS
                     GVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLP
                     VKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITISETLAKFRSRDARDRGLDHA"
     gene            6372..7211
                     /gene="sulI"
                     /label=sulI
     misc_recomb     7218..7258
                     /note="Bxb1 attB42 recognition site; similar to INSD 
                     Accession Number JQ305140"
     regulatory      7289..7479
                     /regulatory_class="terminator"
                     /note="psbA 3UTR; similar to INSD Accession Number 
                     NC_001879"
     misc_feature    7525..7703
                     /note="plastid DNA targeting sequence; similar to inverted 
                     repeat sequence INSD Accession Number Z00044"