LOCUS       Exported               14535 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTBEV-32.11 ic, complete sequence.
ACCESSION   KF623542
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14535)
  AUTHORS   de Fabritus L, Nougairede A, Aubry F, Gould EA, de Lamballerie X.
  TITLE     Attenuation of Tick-Borne Encephalitis Virus Using Large-Scale 
            Random Codon Re-encoding
  JOURNAL   PLoS Pathog. 11 (3), E1004738 (2015)
  PUBMED    25734338
REFERENCE   2  (bases 1 to 14535)
  AUTHORS   de Fabritus L, Nougairede A, de Lamballerie X.
  TITLE     Tick-Borne Encephalitis virus TBEV Oshima 32.11 infectious clone
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 14535)
  AUTHORS   de Fabritus L, Nougairede A, de Lamballerie X.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2013) UMR_D 190 Emergence des Pathologies 
            Virales, Aix-Marseille Univ, 27 Bd Jean Moulin, Marseille 13385, 
            France
REFERENCE   4  (bases 1 to 14535)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14535
                     /organism="Cloning vector pTBEV-32.11 ic"
                     /mol_type="other DNA"
                     /db_xref="taxon:1429999"
     5UTR           1..131
     CDS             132..10376
                     /codon_start=1
                     /product="polyprotein"
                     /label=polyprotein
                     /protein_id="AHB23867.1"
                     /translation="MAGKAILKGKGGGPPRRVSKETAKKTRQSRVRMPNGLVLMRMMGI
                     LWHAVAGTARSPVLKSFWNSVPLKQATAALRKIKKAVSTLMVGLQRRGKRRSAVDWTGW
                     LLVVVLVGVTLAATVRKERDGTTVIRAEGKDAATQVRVENGTCVILATDMGSWCDDSLT
                     YECVTIDQGEEPVDVDCFCRNVDGVYLEYGRCGKQEGSRTRRSVLIPSHAQGDLTGRGH
                     KWLEGDSLRTHLTRVEGWVWKNKILTLAVIAVVWLTVESVVTRVVVVVVLLCLAPVYAS
                     RCTHLENRDFVTGTQGTTRVTLVLELGGCVTITAEGKPSMDVWLDSIYQENPAKTREYC
                     LHAKLSDTKVAARCPTMGPATLAEEHQSGTVCKRDQSDRGWGNHCGLFGKGSIVTCVKA
                     SCEAKKKATGHVYDANKIVYTVKVEPHTGDYVAANETHSGRKTASFTVSSEKTILTMGD
                     YGDVSLLCRVASGVDLAQTVILELDKTSEHLPTAWQVHRDWFNDLALPWKHEGAQNWNN
                     AERLVEFGAPHAVKMDVYNLGDQTGVLLKSLAGVPVAHIDGTKYHLKSGHVTCEVGLEK
                     LKMKGLTYTVCDKTKFTWKRIPTDSGHDTVVMEVAFSGTKPCRIPVRAVAHGSPDVNVA
                     MLITPNPTIENNGGGFIEMQLPPGDNIIYVGELSHQWFQKGSSIGRVFQKTRKGIERLT
                     VIGEHAWDFGSTGGFLTSVGKALHTVLGGAFNSLFGGVGFLPKILVGMALAWLGLNMRN
                     PTMSMSFLLAGGLVLAMTLGVGADVGCAVDTERMELRCGEGLVVWREVSEWYDNYAYYP
                     ETPGALASAIKETFEEGTCGILPQNRLEMAMWRSSVTELNLALAEGDANLTVVVDKFDP
                     TDYRGGIPGLLKKGKDIKVSWKSWGHSMIWSVPEAPRRFMVGIEGSSECPLERRKTGVF
                     TVAEFGVGLRTKVFLDFRQESTHECDTGVMGAAVKNGMAVHTDQSLWMKSVRNDTGTYI
                     VELLVTDLRNCSWPASHTIDNAEVVDSELFLPASLAGPRSWYNRIPGYSEQVKGPWKYS
                     PIRVTREECPGTRVTINADCDKRGASVRSTTESGKVIPEWCCRTCTLPPVTFRTGTDCW
                     YAMEIRPVHDQGGLVRSMVVADNGELLSEGGIPGIVALFVVLEYVIRRRPATGTTAMWG
                     GIVVLALLVTGLVRIESLVRYVVAVGITFHLELGPEIVALTLLQAVFELRVGLLSAFAL
                     RSNLTVREMVTIYFLLLVLELGLPSEGLGALWKWGDALAMGALIFRACTAEEKTGVGLL
                     LMALMTQQDLAIAHYGLMLFLGVASCCSIWKLIRGHREQKGLTWIVPLAGLLGGEGSGV
                     RLLAFWELAVHGRRRSFSEPLTVVGVMLTLASGMMRHTSQEALCALAVASFLLLMLVLG
                     TRKMQLVAEWSGCVEWHPELMNEGGEVSLRVRQDSMGNFHLTELEKEERVMAFWLLAGL
                     AASAFHWSGILGVMGLWTLSEMLRTARRSDLVFSGQGGRERGDRPFEVKDGVYRIFSPG
                     LLWGQRQVGVGYGFKGVLHTMWHVTRGAALSVDDAVVGPYWADVKEDVVCYGGAWSLEE
                     KWKGETVQVHAFPPGRAHEVHQCQPGELLLDTGGRIGAVPIDLAKGTSGSPILNSQGVV
                     VGLYGNGLKTNETYVSSIAQGEAEKSRPNLPPAVIGTGWTAKGQITVLDMHPGSGKTHR
                     VLPELIRQCIDRRLRTLVLAPTRVVLKEMERALSGKRVRFHSPAVGDQQVGGAIVDVMC
                     HATYVNRRLLPQGRQNWEVAIMDEAHWTDPHSIAARGHLYTLAKENKCALVLMTATPPG
                     KSEPFPESNGAISSEEKQIPDGEWRDGFDWITEYEGRTAWFVPSIAKGGIIARTLRQKG
                     KSVICLNSKTFEKDYSRVRDEKPDFVVTTDISEMGANLDVSRVIDGRTNIKPEEVDGRV
                     ELTGTRRVTTASAAQRRGRVGRQEGRTDEYIYSGQCDDDDGGLVQWKEAQILLDNITTL
                     RGPVATFYGPEQDKMPEVAGHFRLTEEKRKHFRHLLTHCDFTPWLAWHVAANVSSVTSR
                     NWTWEGPEENTVDEANGDLVTFRSPNGAERTLRPVWRDARMFREGRDIREFVAYASGRR
                     SFGDVLSGMSGVPELLRHRCVSAMDVFYTLMHEEPGSRAMKMAERDAPEAFLTVVEMMV
                     LGLATLGVVWCFVVRTSISRMMLGTLVLLASLALLWAGGVSYGNMAGVALIFYTLLTVL
                     QPEAGKQRSSDDNKLAYFLLTLCSLAGLVAANEMGFLEKTKADLSTVLWSEHEELRSWE
                     EWTNIDIQPARSWGTYVLVVSLFTPYIIHQLQTKIQQLVNSAVATGAQAMRDLGGGAPF
                     FGVAGHVMALGVVSLVGATPTSLVVGVGLAAFHLAIVVSGLEAELTQRAHKVFFSAMVR
                     NPMVDGDVINPFGEGEAKPALYERKMSLVLAIVLCLMSVVMNRTVPSITEASAVGLAAA
                     GQLLRPEADTLWTMPVACGLSSVVRGSLWGFLPLGHRLWLRASGSRRGGSEGDTLGDLW
                     KRKLNGCTKEEFFAYRRTGILETERDKARELLRRGETNMGLAVSRGTAKLAWLEERGYA
                     TLKGEVVDLGCGRGGWSYYAASRPAVMSVRAYTIGGKGHETPKMVTSLGWNLIKFRAGM
                     DVFSMQPHRADTIMCDIGESNPDAVVEGERTRKVILLMEQWKNRNPTATCVFKVLAPYR
                     PEVIEALHRFQLQWGGGLVRTPFSRNSTHEMYYSTAVTGNIVNSVNIQSRKLLARFGDQ
                     RGPTRVPELDLGVGTRCVVLAEDKVKEKDVQERISALREQYGETWHMDREHPYRTWQYW
                     GSYRTAPTGSAASLINGVVKLLSWPWNAREDVVRMAMTDTTAFGQQRVFKEKVDTKAQE
                     PQPGTKVIMRAVNDWILERLARKSKPRMCSREEFIAKVKSNAALGAWSDEQNRWSSAKE
                     AVEDPAFWQLVDEERERHLAGRCAHCVYNMMGKREKKLGEFGVAKGSRAIWYMWLGSRF
                     LEFEALGFLNEDHWASRGSSGSGVEGISLNYLGWYLKGLSTLEGGLFYADDTAGWDTKV
                     TNADLEDEEQLLRYMEGEHKQLAATIMQKAYHAKVVKVARPSRDGGCIMDVITRRDQRG
                     SGQVVTYALNTLTNIKVQLIRMMEGEGVIEATDAHNPRLFRVERWLRDHGEERLGRMLV
                     SGDDCVVRPVDDRFSRALYFLNDMAKTRKDVGEWEHSVGFSNWEEVPFCSHHFHELVMK
                     DGRALIVPCRDQDELVGRARVSPGCGWSVRETACLSKAYGQMWLLSYFHRRDLRTLGFA
                     ICSAVPVDWVPTGRTTWSIHASGAWMTTEDMLDVWNRVWILDNPFMHGKEKIVEWRDVP
                     YLPKSHDMLCSSLVGRKERAEWARNIWGAVEKVRKMIGQEKFKDYLSCMDRHDLHWELK
                     LESSII"
     CDS             complement(6037..6054)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     3UTR           10377..11100
     ncRNA           11101..11184
                     /product="hepatitis delta ribozyme"
                     /label=ribozyme
                     /ncRNA_class="ribozyme"
     regulatory      11192..11212
                     /regulatory_class="polyA_signal_sequence"
     CDS             11495..11686
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    11788..11928
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(12114..12702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12873..13733)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      13693..14535
                     /regulatory_class="promoter"
                     /gene="pCMV"
     gene            13693..14535
                     /gene="pCMV"
                     /label=pCMV
     enhancer        13938..14317
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14318..14521
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"