LOCUS       Exported                5670 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pTargeT, complete sequence.
ACCESSION   AY540613
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Promega Corporation.
  JOURNAL   (in) PTARGET(TM) MAMMALIAN EXPRESSION SYSTEM TECHINCAL MANUAL. 
            Promega Corporation (2004)
REFERENCE   2  (bases 1 to 5670)
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2004) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5670)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Mammalian expression vector pTargeT"
                     /mol_type="other DNA"
                     /db_xref="taxon:264728"
     regulatory      1..659
                     /regulatory_class="enhancer"
                     /note="Cytomegalovirus immediate early enhancer"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      669..750
                     /regulatory_class="promoter"
                     /note="Cytomegalovirus immediate early promoter"
     misc_feature    890..1022
                     /label=chimeric intron
                     /note="chimeric intron"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(1051..1377)
                     /codon_start=1
                     /product="lacZa"
                     /label=lacZa
                     /protein_id="AAS48583.1"
                     /translation="MTMITPSYLGDTIEYSSLEFAAAVDGTPGKDITRLEASKDPNSPY
                     SESYYNSLAVVLQRRDWENPGVTQLNRLACHVPFASWRNSEEARSDRPSQQLRSLNGEW
                     RSNF"
     operon          join(1066..1226,1362..1499)
                     /operon="lac"
                     /label=lac operon
     regulatory      1229..1248
                     /regulatory_class="promoter"
                     /note="T7 promoter (-17 to +3)"
     promoter        1229..1247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1250..1323
                     /label=multiple cloning region
                     /note="multiple cloning region"
     primer_bind     1344..1367
                     /label=pTargeT(tm) sequencing primer
                     /note="pTargeT(tm) sequencing primer"
     misc_feature    1397..1413
                     /label=lac operator
                     /note="lac operator"
     protein_bind    1397..1413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1421..1451)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1466..1487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      1535..1755
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     polyA_signal    1543..1664
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1798..2252
                     /label=Phage f1 region
                     /note="Phage f1 region"
     rep_origin      1798..2252
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2260..2630
                     /label=SV40 enhancer and early promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2267..2624
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2475..2610
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2528..2593
                     /label=SV40 minimum origin of replication
                     /note="SV40 minimum origin of replication"
     CDS             2675..3469
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /label=neomycin phosphotransferase
                     /protein_id="AAS48584.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3533..3581
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     polyA_signal    3533..3581
                     /note="synthetic polyadenylation signal"
     promoter        3873..3977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3978..4838
                     /codon_start=1
                     /product="b-lactamase"
                     /label=b-lactamase
                     /note="AmpR"
                     /protein_id="AAS48585.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5009..5597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"