LOCUS       Exported                7664 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  P element transformation vector pTARG, complete sequence.
ACCESSION   DQ269206
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Egli D, Yepiskoposyan H, Selvaraj A, Balamurugan K, Rajaram R, 
            Simons A, Multhaup G, Mettler S, Vardanyan A, Georgiev O, Schaffner 
            W.
  TITLE     A family knockout of all four Drosophila metallothioneins reveals a 
            central role in copper homeostasis and detoxification
  JOURNAL   Mol. Cell. Biol. 26 (6), 2286-2296 (2006)
  PUBMED    16508004
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Egli D, Georgiev O, Schaffner W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2005) Institute of Molucular Biology, University 
            of Zurich, Winterthurer Street 190, Zurich 8057, Switerland
REFERENCE   3  (bases 1 to 7664)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="P element transformation vector pTARG"
                     /mol_type="other DNA"
                     /db_xref="taxon:357872"
     misc_feature    1..8
                     /label=Polylinker
                     /note="Polylinker"
     misc_feature    15..67
                     /label=Flippase recombinase target
                     /note="Flippase recombinase target"
     protein_bind    20..67
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    94..127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    128..264
                     /label=SV40 poly(A) addition site
                     /note="SV40 poly(A) addition site"
     polyA_signal    143..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    421..700
                     /label=HSV poly(A) addition site
                     /note="HSV poly(A) addition site"
     polyA_signal    476..523
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      720..945
                     /regulatory_class="promoter"
                     /note="3xP3-hsp70 promoter"
     misc_feature    951..984
                     /label=loxP
                     /note="loxP"
     protein_bind    951..984
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gene            1006..3378
                     /gene="white"
                     /label=white
     CDS             1178..3241
                     /codon_start=1
                     /gene="white"
                     /product="white"
                     /label=white
                     /protein_id="ABB82948.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    3900..3952
                     /label=flippase recombinase target
                     /note="flippase recombinase target"
     protein_bind    3905..3952
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    4086..4671
                     /label=P element 5 end
     misc_feature    4148..4671
                     /label=P element 5 end
                     /note="P element 5 end"
     misc_feature    4901..6931
                     /label=pUC8 plasmid sequence
                     /note="pUC8 plasmid sequence"
     rep_origin      complement(4906..5494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5665..6525)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6526..6630)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7432..7664
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    7581..7659
                     /label=P element 3 end
                     /note="P element 3 end"