LOCUS       Exported                4711 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pTAPKan1, complete sequence.
ACCESSION   FJ360542
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Spirek M, Gregan J, Chen Z.
  TITLE     Yeast tagging vector pTAPKan1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Spirek M, Gregan J, Chen Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2008) Department of Chromosome Biology, University
            of Vienna, Viehmarktgasse 2a, Wien 1030, Austria
REFERENCE   3  (bases 1 to 4711)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Yeast tagging vector pTAPKan1"
                     /mol_type="other DNA"
                     /db_xref="taxon:571181"
     misc_feature    85..693
                     /note="TAP tag; cassette coding for CBP, a TEV protease 
                     cleavage site and ProtA for C terminal tagging"
     CDS             85..162
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             238..258
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      714..901
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            976..2332
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        976..1319
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1320..2129
                     /codon_start=1
                     /gene="kanMX6"
                     /product="KanR"
                     /label=kanMX6
                     /note="kanamycin and geneticin G418 resistance protein"
                     /protein_id="ACJ05373.1"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1320..2129
                     /gene="kanMX6"
                     /label=kanMX6
     terminator      2135..2332
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2437..2455)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2713..3301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3472..4332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ACJ05374.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3472..4332)
                     /gene="bla"
                     /label=bla
     promoter        complement(4333..4437)
                     /gene="bla"
                     /label=AmpR promoter