LOCUS       Exported                3143 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pT7XbG3, complete sequence.
ACCESSION   MH001534
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Kikawada T, Kikuta S.
  TITLE     Expression vector for synthesis of artificial capped RNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Kikawada T, Kikuta S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2018) Division of Biotechnology, Institute of 
            Agrobiological Sciences, National Institute of Agriculture and Food 
            Research Organization, 1-2 Ohwashi, Tsukuba, Ibaraki 305-0851, Japan
REFERENCE   3  (bases 1 to 3143)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Expression vector pT7XbG3"
                     /mol_type="other DNA"
                     /db_xref="taxon:2100818"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            659..937
                     /gene="beta-globin"
                     /label=beta-globin
                     /note="from Xenopus laevis"
     5UTR           659..686
                     /gene="beta-globin"
     misc_feature    687..737
                     /gene="beta-globin"
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3UTR           743..937
                     /gene="beta-globin"
     3UTR           754..879
                     /label=Xenopus globin 3-UTR
                     /note="3-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(955..975)
                     /label=T3
                     /note="T3"
     promoter        complement(955..973)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(994..1010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1018..1034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1042..1072)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1087..1108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1396..1984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2155..3015)
                     /codon_start=1
                     /gene="Amp+"
                     /product="beta-lactamase"
                     /label=Amp+
                     /protein_id="AVY92591.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2155..3015)
                     /gene="Amp+"
                     /label=Amp+
     promoter        complement(3016..3120)
                     /gene="bla"
                     /label=AmpR promoter