LOCUS       Exported                3846 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT7XbG2-AcGFP1 DNA, complete sequence.
ACCESSION   AB255038
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Kikawada T, Kanamori Y, Saito A, Watanabe M, Okuda T.
  TITLE     Expression vector for Xenopus oocyte
  JOURNAL   Published Only in Database (2006)
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Kikawada T, Kanamori Y, Saito A, Watanabe M, Okuda T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2006) Takahiro Kikawada, National Institute of 
            Agrobiological Sciences; Ohwashi 1-2, Tsukuba 305-8634, Japan 
            (E-mail:kikawada@affrc.go.jp, Tel:81-29-838-6087)
REFERENCE   3  (bases 1 to 3846)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          join(1..653,1640..3846)
                     /organism="Cloning vector pBluescript II SK (+)"
                     /mol_type="other DNA"
                     /db_xref="taxon:378729"
     source          1..3846
                     /organism="Cloning vector pT7XbG2-AcGFP1"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:378728"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5UTR           659..687
                     /note="Xenopus beta-globin 5-UTR, sub-cloned from 
                     pXbG-ev1"
     misc_feature    687..699
                     /note="contains multiple cloning site (BgI II, EcoR V, EcoR
                     I)"
     CDS             714..1433
                     /codon_start=1
                     /gene="AcGFP1"
                     /product="AcGFP1, sub-cloned from pIRES2-AcGFP1"
                     /label=AcGFP1
                     /note="GFP derived from Aequorea coerulescens"
                     /protein_id="BAE93141.1"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     gene            714..1433
                     /gene="AcGFP1"
                     /label=AcGFP1
     3UTR           1441..1639
                     /note="Xenopus beta-globin 3-UTR, sub-cloned from pXbG-ev1
                     and poly A tail"
     3UTR           1457..1582
                     /label=Xenopus globin 3-UTR
                     /note="3-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(1658..1676)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1697..1713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1721..1737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1745..1775)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1790..1811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2099..2687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2858..3718)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3719..3823)
                     /gene="bla"
                     /label=AmpR promoter