LOCUS       Exported                4576 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Reverse genetics vector pT7-S, complete sequence.
ACCESSION   KJ648914
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Bergeron E, Zivcec M, Chakrabarti AK, Nichol ST, Albarino CG, 
            Spiropoulou CF.
  TITLE     Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus 
            Reveals a Function for Non-structural Glycoproteins Cleavage by 
            Furin
  JOURNAL   PLoS Pathog. 11 (5), E1004879 (2015)
  PUBMED    25933376
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Bergeron E, Chakrabarti AK, Nichol ST, Albarino CG, Spiropoulou CF.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2014) Viral Special Pathogens Branch, Centers for 
            Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 
            30333, USA
REFERENCE   3  (bases 1 to 4576)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Reverse genetics vector pT7-S"
                     /mol_type="other DNA"
                     /label=derived from Crimean-Congo hemorrhagic fever virus
                     /note="derived from Crimean-Congo hemorrhagic fever virus"
                     /db_xref="taxon:1504879"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     promoter        705..809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             810..1670
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /protein_id="AIN41196.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            810..1670
                     /gene="ampR"
                     /label=ampR
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2687..2704
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        2688..2705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5UTR           2706..2760
                     /label=viral UTR
                     /note="viral UTR"
     CDS             2761..4209
                     /codon_start=1
                     /gene="N"
                     /product="nucleoprotein"
                     /label=N
                     /protein_id="AIN41195.1"
                     /translation="MENKIEVNNKDEMNRWFEEFKKGNGLVDTFTNSYSFCESVPNLDR
                     FVFQMASATDDAQKDSIYASALVEATKFCAPIYECAWVSSTGIVKKGLEWFEKNAGTIK
                     SWDESYTELKVDVPKIEQLTGYQQAALKWRKDIGFRVNANTAALSNKVLAEYKVPGEIV
                     MSVKEMLSDMIRRRNLILNRGGDENPRGPVSHEHVDWCREFVKGKYIMAFNPPWGDINK
                     SGRSGIALVATGLAKLAETEGKGIFDEAKKTVEALNGYLDKHKDEVDRASADSMITNLL
                     KHIAKAQELYKNSSALRAQSAQIDTAFSSYYWLYKAGVTPETFPTVSQFLFELGKQPRG
                     TKKMKKALLSTPMKWGKKLYELFADDSFQQNRIYMHPAVLTAGRISEMGVCFGTIPVAN
                     PDDAAQGSGHTKSILNLRTNTETNNPCAKTIVKLFEVQKTGFNIQDMDIVASEHLLHQS
                     LVGKQSPFQNAYNVKGNATSANII"
     gene            2761..4209
                     /gene="N"
                     /label=N
     3UTR           4210..4377
                     /label=viral UTR
                     /note="viral UTR"
     ncRNA           4378..4471
                     /product="Hepatitis D virus ribozyme"
                     /label=other
                     /ncRNA_class="other"
     regulatory      4472..4576
                     /regulatory_class="terminator"
                     /note="T7 terminator"
     terminator      4530..1
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"