LOCUS       Exported                3727 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT2_T1_72bp-DsRed, complete sequence.
ACCESSION   KM486859
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
            & Systems Biology, Tsinghua National Laboratory for Information 
            Science and Technology, Tsinghua University, Beijing 100084, China
REFERENCE   3  (bases 1 to 3727)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Cloning vector pT2_T1_72bp-DsRed"
                     /mol_type="other DNA"
                     /db_xref="taxon:1579934"
     rep_origin      complement(57..645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(816..1676)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="AIZ66022.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1677..1781)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1808..2263)
                     /direction=LEFT
                     /label=f1 origin
                     /note="f1 origin"
     misc_feature    2441..2648
                     /label=T2(+)T1(+)
                     /note="T2(+)T1(+)"
     protein_bind    2441..2535
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    2441..2457
                     /label=UAS
                     /note="UAS"
     misc_feature    2460..2476
                     /label=UAS
                     /note="UAS"
     misc_feature    2479..2495
                     /label=UAS
                     /note="UAS"
     misc_feature    2498..2514
                     /label=UAS
                     /note="UAS"
     misc_feature    2517..2533
                     /label=UAS
                     /note="UAS"
     misc_feature    2569..2628
                     /label=CMVmini
                     /note="CMVmini"
     regulatory      2662..2671
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2668..3345
                     /codon_start=1
                     /product="DsRed"
                     /label=DsRed
                     /protein_id="AIZ66023.1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     misc_feature    3356..3377
                     /label=FF5
                     /note="FF5"
     misc_feature    3378..3399
                     /label=FF5
                     /note="FF5"
     misc_feature    3400..3421
                     /label=FF5
                     /note="FF5"
     misc_feature    3422..3443
                     /label=FF5
                     /note="FF5"
     polyA_signal    3600..3721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"