LOCUS       Exported                7438 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT2gammaCryK2, complete sequence.
ACCESSION   JN418198
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Loots G, Bergmann A, Hum N, Hu N, Oldenburg C, Khokha M, Ovcharenko 
            I, Harland RM.
  TITLE     Interrogating Distal Muscle Enhancers in Tol2-mediated Xenopus 
            Transgenics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Loots G, Bergmann A, Hum N.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2011) Biosciences & Biotechnology Division, 
            Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore,
            CA 94550, USA
REFERENCE   3  (bases 1 to 7438)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Cloning vector pT2gammaCryK2"
                     /mol_type="other DNA"
                     /db_xref="taxon:1127335"
     regulatory      13..2434
                     /regulatory_class="promoter"
                     /note="Xenopus laevis gamma crystallin lens-specific 
                     promoter (gammaCry)"
     promoter        156..174
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2429..2438
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     mRNA            2435..3335
                     /gene="mKate2"
                     /product="mKate2"
                     /label=mKate2 mRNA
     gene            2435..3335
                     /gene="mKate2"
                     /label=mKate2
     CDS             2435..3133
                     /codon_start=1
                     /gene="mKate2"
                     /product="mKate2"
                     /label=mKate2
                     /note="far red fluorescent protein; excitation max 
                     588/emission max 633"
                     /protein_id="AEX25288.1"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3255..3376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3388..4039
                     /label=Tol2 transposase recombinant sequence 1
                     /note="Tol2 transposase recombinant sequence 1"
     promoter        complement(4185..4203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4430..5068
                     /label=ColE1 Ori
                     /note="ColE1 Ori"
     rep_origin      complement(4461..5049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5220..6080)
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /protein_id="AEX25287.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5220..6080)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(6081..6185)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6531..6549
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    6588..7420
                     /label=Tol2 transposase recombinant sequence 2
                     /note="Tol2 transposase recombinant sequence 2"
     primer_bind     6593..6609
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7281..7415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"