LOCUS       Exported                5906 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pT1NX, complete sequence.
ACCESSION   HM585371
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Steidler L.
  TITLE     pT1NX expression vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Steidler L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2010) Technology Development, ActoGeniX NV, 
            Zwijnaarde, Belgium, Technologiepark 4, Zwijnaarde, OVL 9052, 
            Belgium
REFERENCE   3  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Expression vector pT1NX"
                     /mol_type="other DNA"
                     /note="used in Lactococcus lactis; uses sequence from 
                     cloning vector pIL253"
                     /db_xref="taxon:858216"
     regulatory      1..149
                     /regulatory_class="promoter"
                     /note="P1 promoter from Lactococcus lactis"
     sig_peptide     218..298
                     /gene="usp45"
     CDS             218..298
                     /codon_start=1
                     /gene="usp45"
                     /product="Usp45"
                     /label=usp45
                     /note="signal sequence"
                     /protein_id="ADJ57691.1"
                     /translation="MKKKIISAILMSTVILSAAAPLSGVYA"
     gene            218..298
                     /gene="usp45"
                     /label=usp45
                     /note="from Lactococcus sp."
     CDS             306..908
                     /codon_start=1
                     /gene="spaX"
                     /product="SpaX"
                     /label=spaX
                     /note="protein A anchor"
                     /protein_id="ADJ57690.1"
                     /translation="DPKEEDNNKPGKEDNNKPGKEDNNKPGKEDNNKPGKEDNNKPGKE
                     DNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDGNKPGKEDGN
                     GVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVDKKQPANHADANKA
                     QALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL"
     gene            306..908
                     /gene="spaX"
                     /label=spaX
                     /note="from Staphylococcus aureus"
     terminator      978..1025
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      983..1022
                     /regulatory_class="terminator"
     CDS             1605..2342
                     /codon_start=1
                     /gene="ermAM"
                     /product="ErmAM"
                     /label=ermAM
                     /note="pAMbeta1 resistance protein"
                     /protein_id="ADJ57693.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            1605..2342
                     /gene="ermAM"
                     /label=ermAM
                     /note="from Enterococcus faecalis"
     CDS             2463..2933
                     /codon_start=1
                     /product="transposase Tn3"
                     /label=transposase Tn3
                     /protein_id="ADJ57692.1"
                     /translation="MGKLGSYARQNKLATALGEMGRIEKTLFTLDYISNKAVRRRVQKG
                     LNKGEAINALARTIFFGQRGEFRERALQDQLQRASALNIIINAISVWNTVYMEKAVEEL
                     KARGEFREDLMPYAWPLGWEHINFLGEYKFEGLHDTGQMNLRPLRIKEPFYS"
     rep_origin      3088..5906
                     /label=from Enterococcus faecalis pAMbeta1
                     /note="from Enterococcus faecalis pAMbeta1"