LOCUS       Exported                4850 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSYFP2-sp, complete sequence.
ACCESSION   MH050936
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Zhang JY, Lin GM, Xing WY, Zhang CC.
  TITLE     Diversity of Growth Patterns Probed in Live Cyanobacterial Cells 
            Using a Fluorescent Analog of a Peptidoglycan Precursor
  JOURNAL   Front Microbiol 9, 791 (2018)
  PUBMED    29740419
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Zhang J-Y., Lin G-M., Xing W-Y., Zhang C-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, 
            Wuhan, Hubei 430072, 086
REFERENCE   3  (bases 1 to 4850)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Cloning vector pSYFP2-sp"
                     /mol_type="other DNA"
                     /db_xref="taxon:2201267"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /protein_id="AWK77092.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            201..1061
                     /gene="amp"
                     /label=amp
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2308..2334
                     /label=domain linker
                     /note="domain linker"
     misc_feature    2335..3048
                     /label=super yellow fluorescent protein 2
                     /note="super yellow fluorescent protein 2"
     misc_feature    3049..3078
                     /label=domain linker
                     /note="domain linker"
     misc_feature    3079..3168
                     /label=TwinStrep affinity purification tag
                     /note="TwinStrep affinity purification tag"
     CDS             3085..3108
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3145..3168
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      3179..3226
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      3179..3225
                     /regulatory_class="terminator"
     CDS             3467..4258
                     /codon_start=1
                     /gene="sp"
                     /product="spectinomycin and streptomycin resistance 
                     protein"
                     /label=sp
                     /protein_id="AWK77093.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            3467..4258
                     /gene="sp"
                     /label=sp
     regulatory      4316..4334
                     /regulatory_class="terminator"
     primer_bind     complement(4456..4472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"