LOCUS       Exported                8459 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV57, complete sequence.
ACCESSION   LT727595
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8459)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Mammalian expression vector pSV57"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 1835."
                     /db_xref="taxon:1945120"
     regulatory      1..278
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     promoter        6..335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      186..321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      235..310
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             join(349..594,941..2821)
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     CDS             349..873
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88940.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    349..594
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    595..940
                     /label=intron
                     /note="intron"
     intron          595..940
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    941..2821
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     regulatory      complement(2822..2918)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      2822..2918
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_signal    2844..2978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2982..3014
                     /label=lacO
                     /note="lacO"
     misc_feature    complement(3058..3995)
                     /label=intron
                     /note="intron"
     misc_feature    complement(3996..4198)
                     /label=5UTR
                     /note="5UTR"
     regulatory      complement(4199..4494)
                     /regulatory_class="promoter"
                     /note="SV40 late promoter"
     promoter        4231..4588
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      4250..4531
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      4439..4574
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      4488..4563
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             4602..5126
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88941.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    4602..4847
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    4848..5193
                     /label=intron
                     /note="intron"
     intron          4848..5193
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    5194..6256
                     /label=part of SV40 large t-antigen
                     /note="part of SV40 large t-antigen"
     CDS             5323..5343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6398..6538
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6440..6910)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(6724..7312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7483..8343)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88942.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8344..8448)
                     /gene="bla"
                     /label=AmpR promoter