LOCUS       Exported                9459 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV529HIFNG3, complete sequence.
ACCESSION   LT727498
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Mammalian expression vector pSV529HIFNG3"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2334."
                     /db_xref="taxon:1945113"
     promoter        82..439
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      101..382
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      290..425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      339..414
                     /label=SV40 ori
                     /note="SV40 ori"
     misc_feature    453..981
                     /label=SV40 small t-antigen
                     /note="SV40 small t-antigen"
     misc_feature    453..698
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    699..1048
                     /label=intron
                     /note="intron"
     intron          983..1048
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     misc_feature    1049..2929
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     CDS             1178..1198
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      complement(2930..3026)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      2930..3026
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_signal    2952..3086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3369..3869)
                     /codon_start=1
                     /note="unnamed protein product; hIFNG"
                     /protein_id="SJL88658.1"
                     /translation="MKYTSYILAFQLCIVLGSLGCYCQDPYVKEAENLKKYFNAGHSDV
                     ADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKF
                     FNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRR
                     ASQ"
     misc_feature    complement(3966..4903)
                     /label=intron
                     /note="intron"
     misc_feature    complement(4904..5106)
                     /label=5UTR
                     /note="5UTR"
     regulatory      complement(5107..5402)
                     /regulatory_class="promoter"
                     /note="SV40 late promoter"
     promoter        5139..5496
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      5158..5439
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      5347..5482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      5396..5471
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             5510..6034
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88659.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    5510..5755
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    5756..6101
                     /label=intron
                     /note="intron"
     intron          5756..6101
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    6102..7164
                     /label=part of SV40 large t-antigen
                     /note="part of SV40 large t-antigen"
     CDS             6231..6251
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7306..7446
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7448..7850)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(7632..8220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8391..9251)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88660.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9252..9356)
                     /gene="bla"
                     /label=AmpR promoter