LOCUS       Exported               10612 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV51E6Hy3f22, complete sequence.
ACCESSION   LT727612
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10612)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Mammalian expression vector pSV51E6Hy3f22"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 1983."
                     /db_xref="taxon:1945109"
     promoter        82..439
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      101..382
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      290..425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      339..414
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             join(453..698,1045..2925)
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     CDS             453..977
                     /codon_start=1
                     /note="unnamed protein product; small t-antigen of SV40"
                     /protein_id="SJL88976.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    453..698
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    699..1044
                     /label=intron of large T-antigen of SV40
                     /note="intron of large T-antigen of SV40"
     intron          699..1044
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     CDS             1045..2925
                     /codon_start=1
                     /note="unnamed protein product; large T-antigen of SV40"
                     /protein_id="SJL88977.1"
                     /translation="IPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPK
                     KKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISR
                     HNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIE
                     ESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLK
                     CIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTN
                     RFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYW
                     LFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGE
                     SRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQAR
                     FVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEW
                     KERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSG
                     HETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET"
     regulatory      complement(2926..3022)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      2926..3022
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     polyA_signal    2948..3082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3105..3200)
                     /label=fragment of CH2 domain of mIghg2b
                     /note="fragment of CH2 domain of mIghg2b"
     misc_feature    complement(3201..3318)
                     /label=intron of mIghg2b
                     /note="intron of mIghg2b"
     misc_feature    complement(3319..3363)
                     /label=H4 domain of mIghg2b
                     /note="H4 domain of mIghg2b"
     misc_feature    complement(3364..3506)
                     /label=intron of mIghg2b
                     /note="intron of mIghg2b"
     misc_feature    complement(3507..3551)
                     /label=H3 domain of mIghg2b
                     /note="H3 domain of mIghg2b"
     misc_feature    complement(3552..3694)
                     /label=intron of mIghg2b
                     /note="intron of mIghg2b"
     misc_feature    complement(3695..3739)
                     /label=H2 domain of mIghg2b
                     /note="H2 domain of mIghg2b"
     misc_feature    complement(3740..3882)
                     /label=intron of mIghg2b
                     /note="intron of mIghg2b"
     misc_feature    complement(3883..3933)
                     /label=H1 domain of mIghg2b
                     /note="H1 domain of mIghg2b"
     misc_feature    complement(3934..4324)
                     /label=intron of mIghg2b
                     /note="intron of mIghg2b"
     misc_feature    complement(4325..4615)
                     /label=CH1 domain of mIghg2b
                     /note="CH1 domain of mIghg2b"
     misc_feature    complement(4616..4975)
                     /label=VH domain of mIghg2b
                     /note="VH domain of mIghg2b"
     misc_feature    complement(4976..5032)
                     /label=L domain of mIghg2b
                     /note="L domain of mIghg2b"
     misc_feature    complement(5119..6056)
                     /label=intron of mIghg2b
                     /note="intron of mIghg2b"
     misc_feature    complement(6057..6259)
                     /label=5 UTR of mIghg2b
                     /note="5 UTR of mIghg2b"
     regulatory      complement(6260..6555)
                     /regulatory_class="promoter"
                     /note="SV40 late promoter"
     promoter        6292..6649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      6311..6592
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      6500..6635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      6549..6624
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             6663..7187
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88978.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    6663..6908
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    6909..7254
                     /label=intron
                     /note="intron"
     intron          6909..7254
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    7255..8317
                     /label=part of SV40 large t-antigen
                     /note="part of SV40 large t-antigen"
     CDS             7384..7404
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    8459..8599
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8595..9005)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(8785..9373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9544..10404)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88979.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10405..10509)
                     /gene="bla"
                     /label=AmpR promoter