LOCUS       Exported               11377 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV51E6Hy3, complete sequence.
ACCESSION   LT727611
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11377)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 11377)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11377
                     /organism="Mammalian expression vector pSV51E6Hy3"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 1982."
                     /db_xref="taxon:1945108"
     promoter        82..439
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      101..382
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      290..425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      339..414
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             join(453..698,1045..2925)
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     CDS             453..977
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88973.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    453..698
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    699..1044
                     /label=intron
                     /note="intron"
     intron          699..1044
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    1045..2925
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     regulatory      complement(2926..3022)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      2926..3022
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_signal    2948..3082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3098..3214)
                     /label=3 UTR of mIghg2b
                     /note="3 UTR of mIghg2b"
     misc_feature    complement(3215..3538)
                     /label=CH3 domain of mIghg2b
                     /note="CH3 domain of mIghg2b"
     misc_feature    complement(3539..3635)
                     /label=intron of of mIghg2b gene
                     /note="intron of of mIghg2b gene"
     misc_feature    complement(3636..3965)
                     /label=CH2 domain of mIghg2b
                     /note="CH2 domain of mIghg2b"
     misc_feature    complement(3966..4083)
                     /label=intron of of mIghg2b gene
                     /note="intron of of mIghg2b gene"
     misc_feature    complement(4084..4128)
                     /label=H4 domain of mIghg2b
                     /note="H4 domain of mIghg2b"
     misc_feature    complement(4129..4271)
                     /label=intron of of mIghg2b gene
                     /note="intron of of mIghg2b gene"
     misc_feature    complement(4272..4316)
                     /label=H3 domain of mIghg2b
                     /note="H3 domain of mIghg2b"
     misc_feature    complement(4317..4459)
                     /label=intron of of mIghg2b gene
                     /note="intron of of mIghg2b gene"
     misc_feature    complement(4460..4504)
                     /label=H2 domain of mIghg2b
                     /note="H2 domain of mIghg2b"
     misc_feature    complement(4505..4647)
                     /label=intron of of mIghg2b gene
                     /note="intron of of mIghg2b gene"
     misc_feature    complement(4648..4698)
                     /label=H1 domain of mIghg2b
                     /note="H1 domain of mIghg2b"
     misc_feature    complement(4699..5089)
                     /label=intron of of mIghg2b gene
                     /note="intron of of mIghg2b gene"
     misc_feature    complement(5090..5380)
                     /label=CH1 domain of mIghg2b
                     /note="CH1 domain of mIghg2b"
     misc_feature    complement(5381..5740)
                     /label=VH domain of mIghg2b
                     /note="VH domain of mIghg2b"
     misc_feature    complement(5741..5797)
                     /label=L domain of mIghg2b
                     /note="L domain of mIghg2b"
     misc_feature    complement(5884..6821)
                     /label=intron of of mIghg2b gene
                     /note="intron of of mIghg2b gene"
     misc_feature    complement(6822..7024)
                     /label=5 UTR of mIghg2b
                     /note="5 UTR of mIghg2b"
     regulatory      complement(7025..7320)
                     /regulatory_class="promoter"
                     /note="SV40 late promoter"
     promoter        7057..7414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      7076..7357
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      7265..7400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      7314..7389
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             7428..7952
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88974.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    7428..7673
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    7674..8019
                     /label=intron
                     /note="intron"
     intron          7674..8019
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    8020..9082
                     /label=part of SV40 large t-antigen
                     /note="part of SV40 large t-antigen"
     CDS             8149..8169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    9224..9364
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9360..9770)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(9550..10138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10309..11169)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88975.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11170..11274)
                     /gene="bla"
                     /label=AmpR promoter