LOCUS       Exported                3602 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV25, complete sequence.
ACCESSION   LT727613
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3602)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Mammalian expression vector pSV25"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 1989."
                     /db_xref="taxon:1945089"
     CDS             1..809
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 VP1"
                     /protein_id="SJL88980.1"
                     /translation="FLCLI*MRT*PVEIF*CGKLLLLKLRLLG*LLC*TCIQGHKKLMK
                     MVLENPFKGQIFIFLLLVGNLWSCRVC*QTTGPNILLKL*PQKMLQLTVSR*TLTTRLF
                     WIRIMLIQWSAGFLIQVKMKTLDILEPTQVGKMCLLFCTLLTQQPQCFLMSRVLGPCAK
                     LTACMFLLLTFVGCLPTLLEHSSGRDFPDILKLPLESGL*KTPTQFPFC*VT*LTGGHR
                     GWMGSL*LECPLK*RRLGFMRTQRSFLGIQT**DTLMSLDKPQLECS"
     polyA_signal    750..884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(810..906)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      810..906
                     /regulatory_class="terminator"
                     /note="polyA"
     promoter        complement(1031..1360)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1045..1180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(1056..1131)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     regulatory      complement(1088..1369)
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     misc_feature    1370..3602
                     /label=pAT
                     /note="pAT"
     rep_origin      complement(1590..1995)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(1775..2363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2534..3394)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88981.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3395..3499)
                     /gene="bla"
                     /label=AmpR promoter