LOCUS       Exported                5256 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV23SmTNFrPDI, complete sequence.
ACCESSION   LT727484
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5256)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Mammalian expression vector pSV23SmTNFrPDI"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3411."
                     /db_xref="taxon:1945088"
     misc_feature    1..813
                     /label=part of SV40 VP1
                     /note="part of SV40 VP1"
     polyA_signal    754..888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(814..910)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      814..910
                     /regulatory_class="terminator"
                     /note="polyA"
     misc_feature    complement(911..1462)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(1313..1333)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1463..1529)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1463..1528
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1530..1604)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL88614.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1612..1851)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(1869..1913)
                     /codon_start=1
                     /note="unnamed protein product; rPDIf"
                     /protein_id="SJL88615.1"
                     /translation="MEEDDDQKAVKDEL"
     CDS             complement(1920..2387)
                     /codon_start=1
                     /note="unnamed protein product; mTNF"
                     /protein_id="SJL88616.1"
                     /translation="LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKD
                     NQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDT
                     PEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL"
     misc_feature    complement(2388..2624)
                     /label=pmTNF
                     /note="pmTNF"
     misc_feature    complement(2625..2652)
                     /label=5UTR
                     /note="5UTR"
     promoter        complement(2685..3014)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2699..2834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2710..2785)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     regulatory      complement(2742..3023)
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     misc_feature    3024..5256
                     /label=pAT
                     /note="pAT"
     rep_origin      complement(3245..3647)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(3429..4017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4188..5048)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88617.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5049..5153)
                     /gene="bla"
                     /label=AmpR promoter