LOCUS       Exported                5404 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV23SHTNF, complete sequence.
ACCESSION   LT727630
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5404)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Mammalian expression vector pSV23SHTNF"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2173."
                     /db_xref="taxon:1945074"
     CDS             1..809
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 VP1"
                     /protein_id="SJL89028.1"
                     /translation="FLCLI*MRT*PVEIF*CGKLLLLKLRLLG*LLC*TCIQGHKKLMK
                     MVLENPFKGQIFIFLLLVGNLWSCRVC*QTTGPNILLKL*PQKMLQLTVSR*TLTTRLF
                     WIRIMLIQWSAGFLIQVKMKTLDILEPTQVGKMCLLFCTLLTQQPQCFLMSRVLGPCAK
                     LTACMFLLLTFVGCLPTLLEHSSGRDFPDILKLPLESGL*KTPTQFPFC*VT*LTGGHR
                     GWMGSL*LECPLK*RRLGFMRTQRSFLGIQT**DTLMSLDKPQLECS"
     polyA_signal    750..884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(810..906)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      810..906
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     misc_feature    complement(907..1458)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(1309..1329)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1459..1525)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1459..1524
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL89029.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1609..1924)
                     /label=3 UTR of hTNF
                     /note="3 UTR of hTNF"
     CDS             complement(1925..2398)
                     /codon_start=1
                     /note="unnamed protein product; hTNF mature sequence"
                     /protein_id="SJL89030.1"
                     /translation="VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRD
                     NQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRE
                     TPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL"
     CDS             complement(2399..2626)
                     /codon_start=1
                     /note="unnamed protein product; presequence of hTNF"
                     /protein_id="SJL89031.1"
                     /translation="MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGAT
                     TLFCLLHFGVIGPQREEFPRDLSLISPLAQA"
     misc_feature    complement(2627..2774)
                     /label=5 UTR of hTNF
                     /note="5 UTR of hTNF"
     promoter        complement(2833..3162)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2847..2982
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2858..2933)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     regulatory      complement(2890..3171)
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      complement(3393..3795)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(3577..4165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4336..5196)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL89032.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5197..5301)
                     /gene="bla"
                     /label=AmpR promoter