LOCUS       Exported                4446 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSV009, complete sequence.
ACCESSION   KT373982
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   de Vries SPW., Gupta S, Baig A.
  TITLE     Spontaneous mutations in Campylobacter jejuni strain M1: A 
            cautionary tale
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   de Vries SPW., Gupta S, Baig A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2015) Department of Veterinary Medicine, 
            University of Cambridge, Madingley Road, Cambridge CB3 0ES, United 
            Kingdom
REFERENCE   3  (bases 1 to 4446)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Cloning vector pSV009"
                     /mol_type="other DNA"
                     /db_xref="taxon:1745383"
     misc_feature    complement(20..419)
                     /label=CjM1_0055 upstream (right flank)
                     /note="CjM1_0055 upstream (right flank)"
     primer_bind     22..46
                     /label=pSV009_GCamplif_RV1
                     /note="pSV009_GCamplif_RV1"
     CDS             complement(420..1214)
                     /codon_start=1
                     /product="Kanamycin resistance cassette"
                     /label=Kanamycin resistance cassette
                     /protein_id="ALM23383.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1132..1152
                     /label=pSV009_seq_RV1
                     /note="pSV009_seq_RV1"
     regulatory      complement(1215..1276)
                     /regulatory_class="promoter"
                     /note="KAN promoter"
     regulatory      complement(1328..1392)
                     /regulatory_class="promoter"
                     /note="CAT promoter"
     misc_feature    complement(1399..1798)
                     /label=CjM1_0055_0057 (left flank)
                     /note="CjM1_0055_0057 (left flank)"
     primer_bind     complement(1506..1526)
                     /label=pSV009_seq_FW1
                     /note="pSV009_seq_FW1"
     primer_bind     complement(1775..1798)
                     /label=pSV009_GCamplif_FW1
                     /note="pSV009_GCamplif_FW1"
     misc_feature    1817..1974
                     /label=Lac-Operon
                     /note="Lac-Operon"
     primer_bind     complement(1817..1833)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1975..2074)
                     /label=pBR322
                     /note="pBR322"
     misc_feature    complement(2075..2211)
                     /label=pBR322
                     /note="pBR322"
     misc_feature    complement(2212..4213)
                     /label=pBR322
                     /note="pBR322"
     promoter        2307..2411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2412..3272
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3443..4031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4214..4443
                     /label=Lac-operon
                     /note="Lac-operon"
     protein_bind    4319..4340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4355..4385
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4393..4409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4417..4433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"