LOCUS       Exported                7830 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSUP202, complete sequence.
ACCESSION   AY428809
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Rock JL, Nelson DR.
  TITLE     Identification and characterization of a hemolysin gene cluster in 
            Vibrio anguillarum
  JOURNAL   Infect. Immun. 74 (5), 2777-2786 (2006)
  PUBMED    16622215
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Nelson DR, Denkin SM.
  TITLE     Mobilizable vector plasmid pSUP202
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7830)
  AUTHORS   Nelson DR, Denkin SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2003) Cell and Molecular Biology, University of 
            Rhode Island, 117 Morrill Hall, Kingston, RI 02881, USA
REFERENCE   4  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Cloning vector pSUP202"
                     /plasmid="pSUP202"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:252600"
     promoter        599..627
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             675..1865
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline resistance protein"
                     /label=tet
                     /note="TcR"
                     /protein_id="AAS60099.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            675..1865
                     /gene="tet"
                     /label=tet
     CDS             2504..2695
                     /codon_start=1
                     /gene="rop"
                     /product="Rop regulatory protein"
                     /label=rop
                     /protein_id="AAS60100.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     gene            2504..2695
                     /gene="rop"
                     /label=rop
     misc_feature    2797..2937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3884..4744)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /note="penicillinase; lactamase; ApR"
                     /protein_id="AAS60101.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3884..4744
                     /gene="bla"
                     /label=bla
     promoter        complement(4745..4849)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5052..5154
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5155..5814
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /protein_id="AAS60102.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            5155..5814
                     /gene="cat"
                     /label=cat
     CDS             complement(6666..7037)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            7070..7179
                     /note="incP origin of transfer"