LOCUS       Exported                9816 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle reporter vector pSUN119, complete sequence.
ACCESSION   AY622811
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Argueta C, Yuksek K, Summers M.
  TITLE     Construction and use of GFP reporter vectors for analysis of 
            cell-type-specific gene expression in Nostoc punctiforme
  JOURNAL   J. Microbiol. Methods 59 (2), 181-188 (2004)
  PUBMED    15369854
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Argueta C, Yuksek K, Summers ML.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St.,
            Northridge, CA 91330-8303, USA
REFERENCE   3  (bases 1 to 9816)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Shuttle reporter vector pSUN119"
                     /mol_type="other DNA"
                     /db_xref="taxon:282193"
     CDS             complement(185..934)
                     /codon_start=1
                     /gene="gfp"
                     /product="green fluorescent protein"
                     /label=gfp
                     /note="promoterless"
                     /protein_id="AAX31730.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKYEFTGRRFTTS"
     gene            complement(185..934)
                     /gene="gfp"
                     /label=gfp
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    997..1033
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      1196..1286
                     /regulatory_class="terminator"
                     /note="T7"
     terminator      1215..1262
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1311..1327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1335..1351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1359..1389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1404..1425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      1531..2411
                     /label=ColE1 origin of replication for use in E. coli
                     /note="ColE1 origin of replication for use in E. coli"
     rep_origin      complement(1713..2301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2800..3594)
                     /codon_start=1
                     /gene="npt"
                     /product="neomycin phosphotransferase"
                     /function="neomycin and kanamycin resistance"
                     /label=npt
                     /protein_id="AAX31731.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(2800..3594)
                     /gene="npt"
                     /label=npt
     rep_origin      3958..9816
                     /label=pDC1 cyanobacterial origin of replication
                     /note="pDC1 cyanobacterial origin of replication"