LOCUS       Exported               11715 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSUC, complete sequence.
ACCESSION   MG651646
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Obermaier S, Fuertges L.
  TITLE     Biosynthesis of dimeric naphthopyrones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Obermaier S, Fuertges L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2017) Pharmaceutical Chemistry, University 
            Freiburg, Fahnenbergplatz, Freiburg 79085, Germany
REFERENCE   3  (bases 1 to 11715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Cloning vector pSUC"
                     /mol_type="other DNA"
                     /note="derived from Aspergillus autonomously replicating 
                     vector"
                     /db_xref="taxon:2212376"
     regulatory      4..292
                     /regulatory_class="terminator"
     CDS             715..1740
                     /codon_start=1
                     /gene="hph"
                     /product="hygromycin B resistance protein"
                     /label=hph
                     /note="HPH"
                     /protein_id="AWO14301.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLEPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            715..1740
                     /gene="hph"
                     /label=hph
     regulatory      1359..7079
                     /regulatory_class="replication_regulatory_region"
     regulatory      1619..1942
                     /regulatory_class="promoter"
     protein_bind    complement(1812..1845)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(2315..2331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2601..3189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3313..4173)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4174..4265)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4558..4591
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      4920..10146
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     regulatory      10437..11702
                     /regulatory_class="promoter"