LOCUS       Exported                4419 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pSU7 DNA, complete sequence.
ACCESSION   AB246757
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Nagano Y, Takao S, Kudo T, Iizasa E, Anai T.
  TITLE     Yeast-based recombineering of DNA fragments into plant 
            transformation vectors by one-step transformation
  JOURNAL   Plant Cell Rep. 26 (12), 2111-2117 (2007)
  PUBMED    17680244
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Nagano Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2006) Yukio Nagano, Saga University, Analytical 
            Research Center for Experimental Sciences; Honjo-machi 1, Saga 
            840-8502, Japan (E-mail:nagano@cc.saga-u.ac.jp, Tel:81-952-28-8898, 
            Fax:81-952-28-8896)
REFERENCE   3  (bases 1 to 4419)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Shuttle vector pSU7"
                     /mol_type="other DNA"
                     /db_xref="taxon:366494"
     CDS             205..999
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin phosphotransferase II"
                     /label=nptII
                     /protein_id="BAF75368.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            205..999
                     /gene="nptII"
                     /label=nptII
     rep_origin      1063..1943
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2547..2762
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2763..3566
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine-5-phosphate decarboxylase"
                     /label=URA3
                     /protein_id="BAF75369.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            2763..3566
                     /gene="URA3"
                     /label=URA3
     rep_origin      3722..4310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"