LOCUS       Exported                3724 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSU30 DNA, complete sequence.
ACCESSION   AB759085
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Goto K, Nagano Y.
  TITLE     Ultra-low background DNA cloning system
  JOURNAL   PLoS ONE 8 (2), E56530 (2013)
  PUBMED    23409191
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Nagano Y, Goto K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2012) Contact:Yukio Nagano Saga University, 
            Analytical Research Center for Experimental Sciences; 1 Honjo-machi,
            Saga 840-8502, Japan
REFERENCE   3  (bases 1 to 3724)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Cloning vector pSU30"
                     /mol_type="other DNA"
                     /db_xref="taxon:1247385"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="Amp"
                     /product="ampicillin resistant protein"
                     /label=Amp
                     /protein_id="BAM83849.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            132..992
                     /gene="Amp"
                     /label=Amp
     misc_feature    1030..1533
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        1543..1823
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             1824..2498
                     /codon_start=1
                     /gene="TRP1"
                     /product="phosphoribosylanthranilate isomerase"
                     /label=TRP1
                     /protein_id="BAM83850.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     gene            1824..2498
                     /gene="TRP1"
                     /label=TRP1
     primer_bind     complement(2651..2667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2675..2691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2699..2729)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2744..2765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3053..3641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"