LOCUS       Exported                4398 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSU0 DNA, complete sequence.
ACCESSION   AB215109
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Iizasa E, Nagano Y.
  TITLE     Highly efficient yeast-based in vivo DNA cloning of multiple DNA 
            fragments and the simultaneous construction of yeast/ Escherichia 
            coli shuttle vectors
  JOURNAL   BioTechniques 40 (1), 79-83 (2006)
  PUBMED    16454044
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Iizasa E, Nagano Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2005) Contact:Eiichi Iizasa Division of Molecular
            and Cellular Immunoscience, Department of Biomolecular Sciences 
            Faculty of Medicine, Saga University; 1-1, 5 chome, Nabeshima, Saga 
            City, Saga 849-8501, Japan
REFERENCE   3  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Cloning vector pSU0"
                     /mol_type="other DNA"
                     /db_xref="taxon:330877"
     CDS             36..896
                     /codon_start=1
                     /gene="Amp"
                     /product="ampicillin resistant protein"
                     /label=Amp
                     /protein_id="BAE72674.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            36..896
                     /gene="Amp"
                     /label=Amp
     rep_origin      1042..1922
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2526..2741
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2742..3545
                     /codon_start=1
                     /gene="ura3"
                     /product="orotidine-5-phosphate decarboxylase"
                     /label=ura3
                     /protein_id="BAE72675.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            2742..3545
                     /gene="ura3"
                     /label=ura3
     rep_origin      3701..4289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"