LOCUS       Exported                7784 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSTH1-GFP DNA, complete sequence.
ACCESSION   AB078779
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Yamashita M, Hojo M.
  TITLE     Transgenic zebrafish overexpressing HSP70
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Yamashita M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2002) Michiaki Yamashita, National Research 
            Institute of Fisheries Science; 2-12-4 Fukuura, Kanazawa-ku, 
            Yokohama, Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, 
            Tel:81-45-788-7665, Fax:81-45-788-5001)
REFERENCE   3  (bases 1 to 7784)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Cloning vector pSTH1-GFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:185213"
     polyA_signal    390..524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      649..1104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             1392..2252
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     protein_bind    complement(2354..2387)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      2465..3053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3555..3571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      3910..4243
                     /regulatory_class="promoter"
                     /note="rainbow trout heat shock cognate protein HSC70 
                     promoter"
     CDS             4399..6366
                     /codon_start=1
                     /product="platyfish HSP70-1 with S-tag"
                     /label=platyfish HSP70-1 with S-tag
                     /note="S-tag sequence from Met-1 to Ala-17"
                     /protein_id="BAB88643.1"
                     /translation="MKETAAAKFERQHMDSAMASAKGISIGIDLGTTYSCVGVFQHGKV
                     EIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFDAKRLIGRRFDDLVVQS
                     DMKLWPFKVINDNGKPKVQVEYKGEIKTFCPEEISSMVLVKMREIAEAFLGQRVSNAVI
                     TVPAYFNDSQRQATKDAGVISGLNVLRIINEPTAAAIAYGLDKGKRGERNVLIFDLGGG
                     TFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHFVEEFKRKHKKDISQNKRAVRR
                     LRTACERAKRTLSSSTQASIEIDSLFEGIDFYTSITRARFEELNSDLFRGTLEPVEKAL
                     QDAKLDKSKIHEVVLVGGSTRIPKIQKLLQDFFNGRELNKSINPDEAVAYGAAVQAAIL
                     MGDTSENVQDLLLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKQTQIFSTYSDNQPGVL
                     IQVYEGERAMTKDNNLLGKFELSGLPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKE
                     NKITITNDKGRLSKDEIEKMVQDADKYKAEDDQQREKIAAKNSLESYAYHMKSSVEDDA
                     LKGKISEEEKKLVIDKCNQTISWLENNQLAEKEEYEHQQNELERVCKPVVAKLYQGAAA
                     GSSSSQAGGATQGPTVEEVD"
     CDS             4402..4446
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     regulatory      6465..6470
                     /regulatory_class="polyA_signal_sequence"
     regulatory      6471..7040
                     /regulatory_class="promoter"
                     /note="human cytomegalovirus CMV promoter"
     enhancer        6511..6814
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6815..7018
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7057..7066
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7063..7782
                     /codon_start=1
                     /product="green fluorescent protein GFP"
                     /label=green fluorescent protein GFP
                     /protein_id="BAB88644.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"