LOCUS       Exported                8126 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSTAR, complete sequence.
ACCESSION   AF047654
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Zeng Q, Tan YH, Hong W.
  TITLE     A single plasmid vector (pSTAR) mediating efficient 
            tetracycline-induced gene expression
  JOURNAL   Anal. Biochem. 259 (2), 187-194 (1998)
  PUBMED    9618196
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Zeng Q, Tan YH, Hong W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-1998) Membrane Biology Laboratory, Institute of 
            Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, 
            Singapore
REFERENCE   3  (bases 1 to 8126)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Expression vector pSTAR"
                     /plasmid="pSTAR"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:73270"
     protein_bind    14..284
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        317..354
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    482..616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1187..1321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1336..2340)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the reverse tetracycline repressor 
                     rTetR with the C-terminal activation domain of herpes 
                     simplex virus VP16"
                     /label=rtTA
                     /note="In the Tet-On(R) system, rtTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIE
                     GLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHAD
                     ALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        complement(2468..2671)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2672..3051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3155..3484
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3335..3470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3841..4635
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     intron          5056..5121
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5251..5271
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5696..5830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5931..6035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6036..6896
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7067..7655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"