LOCUS       Exported                4203 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Knockout delivery shuttle vector pST-KO, complete sequence.
ACCESSION   KC153037
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK.
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in mycobacteria
  JOURNAL   Appl. Environ. Microbiol. 79 (5), 1718-1729 (2013)
  PUBMED    23315736
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2012) Signal Transduction Lab-I, National 
            Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, Delhi 
            110067, India
REFERENCE   3  (bases 1 to 4203)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ClustalW v. 2.1
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Knockout delivery shuttle vector pST-KO"
                     /mol_type="other DNA"
                     /db_xref="taxon:1283732"
     misc_feature    15..63
                     /label=MCS
                     /note="MCS"
     rep_origin      71..854
                     /label=oriE
                     /note="oriE"
     rep_origin      112..700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    875..3120
                     /label=Phsp60-sacB
                     /note="Phsp60-sacB"
     CDS             3204..14
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=hygromycin B phosphotransferase
                     /protein_id="AGE45666.1"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPDPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGRLTLHR"