LOCUS       Exported                4234 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Unidentified cloning vector pSSD3, complete sequence.
ACCESSION   AB007632
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Kato S, Saeki M.
  TITLE     Signal sequence detection vector pSSD3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Kato S, Saeki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-1997) Contact:Seishi Kato Research Institute of 
            National Rehabilitation Center for the Disabled, Department of 
            Rehabilitation Engineering; 4-1 Namiki, Tokorozawa, Saitama 
            359-8555, Japan
REFERENCE   3  (bases 1 to 4234)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="unidentified cloning vector"
                     /plasmid="pSSD3"
                     /mol_type="other DNA"
                     /label=Signal sequence detection vector pSSD3
                     /note="Signal sequence detection vector pSSD3"
                     /db_xref="taxon:45196"
     promoter        17..346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    30..240
                     /label=SV40 early promoter
                     /note="SV40 early promoter"
     rep_origin      197..332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    279
                     /label=SV40 origin
                     /note="SV40 origin"
     intron          385..481
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     525..541
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        549..567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    549..565
                     /label=T7 RNA polymerase promoter
                     /note="T7 RNA polymerase promoter"
     misc_feature    581..586
                     /note="SmaI site for cloning of a target cDNA fragment 
                     encoding a putative signal sequence"
     misc_feature    588..593
                     /note="PmaCI site for cloning of a target cDNA fragment 
                     encoding a putative signal sequence"
     misc_feature    595..600
                     /note="EcoRV site for cloning of a target cDNA fragment 
                     encoding a putative signal sequence"
     misc_feature    600..1427
                     /note="cDNA fragment encoding human urokinase C-terminal 
                     protease domain"
     CDS             complement(1028..1039)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(1490..1508)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    1510..1644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1942..2530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2701..3561
                     /label=beta-lactamase gene
                     /note="beta-lactamase gene"
     CDS             complement(2701..3561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3562..3666)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    3693..4148
                     /label=f1 phage IG region
                     /note="f1 phage IG region"
     rep_origin      complement(3693..4148)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4166..4182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"